| Judul | An In Vitro Examination of Whether Kratom Extracts Enhance the Cytotoxicity of Low-Dose Doxorubicin against A549 Human Lung Cancer CellsV / Asep Bayu |
| Pengarang | Asep Bayu Dasilva Primarindu Nuswantari Siti Irma Rahmawati Firmansyah Karim Jonathan Ardhianto Panggabean Ni Luh Putu Indi Dharmayanti Dwi Wahyu Indriani Peni Ahmadi Rendi Witular Masteria Yunovilsa Putra |
| EDISI | Vol. 29(6), 1404 |
| Penerbitan | Switzerland : Creative Commons Attribution License (CC BY), 2024 |
| Deskripsi Fisik | 17 :ill |
| Subjek | KRATOM MITRAGYNA SPECIOSA ALKALOID MITRAGYNINE CYTOTOXICITY ANTICANCER |
| Abstrak | Doxorubicin is an effective chemotherapeutic agent in the treatment of solid hematological and non-hematological carcinoma. However, its long-term usage could result in side effects, such as cardiomyopathy, chronic heart failure, neurotoxicity and cancer cell resistance. In this study, we reported the sensitivity enhancement of A549 human lung cancer cells on doxorubicin at a low dose (0.1 ppm) in combination with 10–60 ppm of crude and alkaloid extracts derived from the leaves of Kratom (Mitragyna speciosa (Korth.) Havil. Rubiaceae). A549 cancer cell lines were insensitive to the crude extract containing low mitragynine (MG) (4–5%), while these cells were moderately inhibited by the alkaloid extract containing 40–45% MG (IC50 of 48–55 ppm). The alkaloid extract was found to inhibit A549 cancer cells via apoptosis as suggested by the higher relative fluorescence intensity with Annexin compared to that in propidium iodide (PI), i.e., a positive Annexin and a negative PI. The combination of crude extract and do |
| Bentuk Karya | Tidak ada kode yang sesuai |
| Target Pembaca | Tidak ada kode yang sesuai |
| Lokasi Akses Online | https://doi.org/10.3390/molecules29061404 |
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|---|---|---|---|---|
| ARTVET2496 | ARTVET2496 ASE a | Dapat dipinjam | Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Ruang Baca Umum | Tersedia |
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| 250 | # | # | $a Vol. 29(6), 1404 |
| 260 | # | # | $a Switzerland :$b Creative Commons Attribution License (CC BY),$c 2024 |
| 300 | # | # | $a 17 : $b ill |
| 520 | # | # | $a Doxorubicin is an effective chemotherapeutic agent in the treatment of solid hematological and non-hematological carcinoma. However, its long-term usage could result in side effects, such as cardiomyopathy, chronic heart failure, neurotoxicity and cancer cell resistance. In this study, we reported the sensitivity enhancement of A549 human lung cancer cells on doxorubicin at a low dose (0.1 ppm) in combination with 10–60 ppm of crude and alkaloid extracts derived from the leaves of Kratom (Mitragyna speciosa (Korth.) Havil. Rubiaceae). A549 cancer cell lines were insensitive to the crude extract containing low mitragynine (MG) (4–5%), while these cells were moderately inhibited by the alkaloid extract containing 40–45% MG (IC50 of 48–55 ppm). The alkaloid extract was found to inhibit A549 cancer cells via apoptosis as suggested by the higher relative fluorescence intensity with Annexin compared to that in propidium iodide (PI), i.e., a positive Annexin and a negative PI. The combination of crude extract and doxorubicin sensitized A549 cancer cells to doxorubicin by 1.3 to 2.4 times, while the combination with the alkaloid induced a 2.6- to 3.4-fold increase in sensitivity. The calculated combination index (CI) for doxorubicin with the crude and alkaloid extracts was 0.6 and 0.3, respectively, showing potential synergistic combinations to reduce the level of dosage of doxorubicin used in chemotherapy. In addition, the synergistic enhancement effect of crude extract on the cytotoxic activity of doxorubicin provides insights into the plausibility of non-alkaloids to influence the biological activities of Kratom. |
| 650 | # | 4 | $a ALKALOID |
| 650 | # | 4 | $a ANTICANCER |
| 650 | # | 4 | $a CYTOTOXICITY |
| 650 | # | 4 | $a KRATOM |
| 650 | # | 4 | $a MITRAGYNA SPECIOSA |
| 650 | # | 4 | $a MITRAGYNINE |
| 700 | 0 | # | $a Dasilva Primarindu Nuswantari |
| 700 | 0 | # | $a Dwi Wahyu Indriani |
| 700 | 0 | # | $a Firmansyah Karim |
| 700 | 0 | # | $a Jonathan Ardhianto Panggabean |
| 700 | 0 | # | $a Masteria Yunovilsa Putra |
| 700 | 0 | # | $a Ni Luh Putu Indi Dharmayanti |
| 700 | 0 | # | $a Peni Ahmadi |
| 700 | 0 | # | $a Rendi Witular |
| 700 | 0 | # | $a Siti Irma Rahmawati |
| 856 | # | # | $a https://doi.org/10.3390/molecules29061404 |
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| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | ARTVET2496.pdf | ARTVET2496 | Baca Online |
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