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Judul Molecular detection of bat coronaviruses in three bat species in Indonesia / Ni Luh Putu Indi Dharmayanti
Pengarang Ni Luh Putu Indi Dharmayanti
Diana Nurjanah
Harimurti Nuradji
Ibnu Maryanto
Indra Exploitasia
Risa Indriani
EDISI Vol. 22(6):e70, November 2021
Penerbitan Korea : Korean Society of Veterinary Science, 2021
Deskripsi Fisik 12 :ill
Subjek CORONAVIRUS
ZOONOSES
BATS
INDONESIA
Abstrak Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.
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Lokasi Akses Online https://doi.org/10.4142/jvs.2021.22.e70

 
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245 1 # $a Molecular detection of bat coronaviruses in three bat species in Indonesia /$c Ni Luh Putu Indi Dharmayanti
250 # # $a Vol. 22(6):e70, November 2021
260 # # $a Korea :$b Korean Society of Veterinary Science,$c 2021
300 # # $a 12 : $b ill
520 # # $a Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.
650 # 4 $a BATS
650 # 4 $a CORONAVIRUS
650 # 4 $a ZOONOSES
651 # 4 $a INDONESIA
700 0 # $a Diana Nurjanah
700 0 # $a Harimurti Nuradji
700 0 # $a Ibnu Maryanto
700 0 # $a Indra Exploitasia
700 0 # $a Risa Indriani
856 # # $a https://doi.org/10.4142/jvs.2021.22.e70
990 # # $a ARTVET2503
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