| Judul | Dual anti-inflammatory activities of COX-2/5-LOX driven by kratom alkaloid extracts in lipopolysaccharide-induced RAW 264.7 cells / Siti Irma Rahmawati |
| Pengarang | Siti Irma Rahmawati Mutia Hardhiyuna Dwi Wahyu Indriani Febby Nurdiya Ningsih A’liyatur Rosyidah Firdayani Firdayani Peni Ahmadi Masteria Yunovilsa Putra |
| EDISI | Scientific Reports, 2024, ? 1 |
| Penerbitan | Jawa Barat,Indonesia : National Research and Innovation Agency (BRIN), 2024 |
| Deskripsi Fisik | 13 :ill |
| Subjek | MITRAGYNA SPECIOSA CELLULAR OXIDATIVE STRESS DUAL INHIBITION COX-2/5-LOX ANTI-INFLAMMATORY |
| Abstrak | Cyclooxygenase (COX) and lipoxygenase (LOX) enzymes play a pivotal role in producing proinflammatory eicosanoids, including prostaglandins (PGs) and Leukotrienes (LTs), in the inflammation process. Mitragynine is a primary alkaloid contained in the kratom’s leaves and has been reported to show anti-inflammatory activity by suppressing COX-2 mRNA translation to lowering PGs synthesis. In this study, the Kratom’s alkaloid extract Containing ~46% mitragynine was found to exhibit dual inhibition activity towards COX-2/5-LOX enzymes at concentrations below 25 ppm in the LPS-induced RAW 264.7 macrophage cells. At these levels, no cell toxicity was observed while the cells became death (e.g., 10–46% viability at 50–100 ppm) and only COX-2 inhibition activity was observed after exposed with more than 25 ppm of alkaloid extract. In contrast, the methanolic-crude extract of Kratom’s leaf Containing ~ 5% mitragynine showed no inhibition toward COX-2/5-LOX enzymes and did not toxic onto the cells, even after treated at 1 |
| Bentuk Karya | Tidak ada kode yang sesuai |
| Target Pembaca | Tidak ada kode yang sesuai |
| Lokasi Akses Online | DOI: 10.1038/s41598-024-79229-x |
| No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
|---|---|---|---|---|
| ARTVET2512 | ARTVET2512 SIT d | Dapat dipinjam | Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Ruang Baca Umum | Tersedia |
| Tag | Ind1 | Ind2 | Isi |
| 001 | INLIS000000000019339 | ||
| 005 | 20250310012932 | ||
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| 245 | 1 | # | $a Dual anti-inflammatory activities of COX-2/5-LOX driven by kratom alkaloid extracts in lipopolysaccharide-induced RAW 264.7 cells /$c Siti Irma Rahmawati |
| 250 | # | # | $a Scientific Reports, 2024, ? 1 |
| 260 | # | # | $a Jawa Barat,Indonesia :$b National Research and Innovation Agency (BRIN),$c 2024 |
| 300 | # | # | $a 13 : $b ill |
| 520 | # | # | $a Cyclooxygenase (COX) and lipoxygenase (LOX) enzymes play a pivotal role in producing proinflammatory eicosanoids, including prostaglandins (PGs) and Leukotrienes (LTs), in the inflammation process. Mitragynine is a primary alkaloid contained in the kratom’s leaves and has been reported to show anti-inflammatory activity by suppressing COX-2 mRNA translation to lowering PGs synthesis. In this study, the Kratom’s alkaloid extract Containing ~46% mitragynine was found to exhibit dual inhibition activity towards COX-2/5-LOX enzymes at concentrations below 25 ppm in the LPS-induced RAW 264.7 macrophage cells. At these levels, no cell toxicity was observed while the cells became death (e.g., 10–46% viability at 50–100 ppm) and only COX-2 inhibition activity was observed after exposed with more than 25 ppm of alkaloid extract. In contrast, the methanolic-crude extract of Kratom’s leaf Containing ~ 5% mitragynine showed no inhibition toward COX-2/5-LOX enzymes and did not toxic onto the cells, even after treated at 100 ppm. The alkaloid extract suppressed Several antiinflammation parameters, including ROS (64% reduction at 25 ppm), NO (30% reduction at 25 ppm), TNF-? (~ 50% reduction at 25 ppm), and IL-6 production (60% reduction at 6.25 ppm). In silico molecular studies indicated strong binding affinity of Kratom alkaloids to COX-2 and 5-LOX active sites, supporting the Kratom’s alkaloids to have great potential dual inhibition activity towards COX2/5-LOX enzymes and to be developed as a safer NSAIDs with fewer side effects. |
| 650 | # | 4 | $a ANTI-INFLAMMATORY |
| 650 | # | 4 | $a CELLULAR OXIDATIVE STRESS |
| 650 | # | 4 | $a DUAL INHIBITION COX-2/5-LOX |
| 650 | # | 4 | $a MITRAGYNA SPECIOSA |
| 700 | 0 | # | $a A’liyatur Rosyidah |
| 700 | 0 | # | $a Dwi Wahyu Indriani |
| 700 | 0 | # | $a Febby Nurdiya Ningsih |
| 700 | 0 | # | $a Firdayani Firdayani |
| 700 | 0 | # | $a Masteria Yunovilsa Putra |
| 700 | 0 | # | $a Mutia Hardhiyuna |
| 700 | 0 | # | $a Peni Ahmadi |
| 856 | # | # | $a DOI: 10.1038/s41598-024-79229-x |
| 990 | # | # | $a ARTVET2512 |
| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | ARTVET2512.pdf | ARTVET2512 | Baca Online |
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