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Judul Nanokitosan Efektif Menekan Jumlah Bakteri Gram Negatif dan Gram Positif : (NANOCITOSAN EFFECTIVELY SUPPRESSES BACTERIA GRAM NEGATIVE AND GRAM POSITIVE CAUSES OF ENDOMETRITIS IN FRIESIAN HOLSTEIN COW IN VITRO) / Sinaga Edelina
Pengarang Sinaga Edelina
Karja Kurniani, Ni Wayan
Andriani
Amrozi
EDISI Jurnal Veteriner
Penerbitan 2021
Deskripsi Fisik Vol. 22 No. 2 : 198 - 206
Subjek bacteria
endometritis
FH cows
in vitro
nanochitosan
endometritis
Abstrak This study was conducted to isolate and identify the caused bacteria of endometritis in Frisian Holstein (FH) and assessed the effectiveness of nanochitosan against bacterial endometritis in vitro. Sample was collected from six FH cows with subclinical endometritis was examined by ultrasonography, then was calculated the number of colonies, isolation and identification of bacteria. Nanochitosan was calculated the protein content and made into neutral pH with concentrations of 0,5%, 1% and 2%. The results showed that the highest total plate count in the endometrial fluid was founded at number 626 with 4,7 ± 0,6 Log cells/mL and the lowest was found at number 532 with 3,3 ± 0,8 Log cells/mL. Bacteria in the Endometritis fluid was founded Gram-negative bacteria were Escherichia coli, Pseudomonas aeruginosa, and Klebsiella sp. Furthermore, Gram-positive bacteria were founded Staphylococcus aureus, Bacillus sp. and S. pyogenes. The challenge test of nanochitosan 1% and 2% with E. coli showed insignifica
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Lokasi Akses Online 10.19087/jveteriner.2021.22.2.198

 
No Barcode No. Panggil Akses Lokasi Ketersediaan
00000020607 ARTVET2385 Dapat dipinjam Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Ruang Baca Umum Tersedia
Tag Ind1 Ind2 Isi
001 INLIS000000000018071
005 20220224122738
007 ta
008 220224################|##########|#|##
035 # # $a 0010-0222000045
084 # # $a ARTVET2385
100 1 # $a Sinaga Edelina
245 1 # $a Nanokitosan Efektif Menekan Jumlah Bakteri Gram Negatif dan Gram Positif : $b (NANOCITOSAN EFFECTIVELY SUPPRESSES BACTERIA GRAM NEGATIVE AND GRAM POSITIVE CAUSES OF ENDOMETRITIS IN FRIESIAN HOLSTEIN COW IN VITRO) /$c Sinaga Edelina
250 # # $a Jurnal Veteriner
260 # # ,$c 2021
300 # # $a Vol. 22 No. 2 : 198 - 206
520 # # $a This study was conducted to isolate and identify the caused bacteria of endometritis in Frisian Holstein (FH) and assessed the effectiveness of nanochitosan against bacterial endometritis in vitro. Sample was collected from six FH cows with subclinical endometritis was examined by ultrasonography, then was calculated the number of colonies, isolation and identification of bacteria. Nanochitosan was calculated the protein content and made into neutral pH with concentrations of 0,5%, 1% and 2%. The results showed that the highest total plate count in the endometrial fluid was founded at number 626 with 4,7 ± 0,6 Log cells/mL and the lowest was found at number 532 with 3,3 ± 0,8 Log cells/mL. Bacteria in the Endometritis fluid was founded Gram-negative bacteria were Escherichia coli, Pseudomonas aeruginosa, and Klebsiella sp. Furthermore, Gram-positive bacteria were founded Staphylococcus aureus, Bacillus sp. and S. pyogenes. The challenge test of nanochitosan 1% and 2% with E. coli showed insignificant results when compared with positive controls (P>0,05). Nanochitosan with all concentrations can reduced number colonies of P. aeruginosa and Bacillus sp. significantly compared with negative controls (P<0,05). The challenge test of nano chitosan 0,5% and 2% were able to reduced the number of S. aureus and Klebsiella sp. colonies but could not reduce of S. pyogenes. These findings showed treatment of nanochitosan as an antibacterial is able to reduce the number of E. coli, Klebsiella sp. and P. aeruginosa and Bacillus sp. and S. aureus
600 # 4 $a endometritis
650 # 4 $a bacteria
650 # 4 $a endometritis
650 # 4 $a FH cows
650 # 4 $a in vitro
650 # 4 $a nanochitosan
700 1 # $a Amrozi
700 1 # $a Andriani
700 1 # $a Karja Kurniani, Ni Wayan
856 # # $a 10.19087/jveteriner.2021.22.2.198
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