| Judul | Identifikasi Subtipe Virus Avian Influenza Isolat Legok Menggunakan Polymerase Chain Reaction |
| Pengarang | Enuh Raharjo Jusa Pudjiatmoko Emilia Bahruddin Syahroni Hasibuan Sri Murni Astuti |
| EDISI | Vol. 10, p. 1-4 |
| Penerbitan | Bogor BBPMSOH 2004 |
| Deskripsi Fisik | 2 tables.:7 ref.: Summary (En) |
| ISBN | 0852-9612 |
| Subjek | PCR -- Electrophoresis -- Layer chickens -- ISOLATION -- JAVA -- Avian Influenza Virus -- Gene -- Agglutinins |
| Catatan | The virology laboratory of Veterinary Drug Assay Laboratory isolated a virus which caused respiratory clinical signs in chicken from a layer farm, in Legok, Tangerang. The clinical signs of the disease were very similar to the avian influenza. The virus isolate was then identified by RT-PCR using primer NP-1200f and NP-1529r that amplified nucleoprotein gene of all influenza virus of poultry, man, pig and horse, and using 15 pair of H1-15 primer that amplified haemagglutinin antigen (HA) of avian subtype. The result of RT-PCR showed that both of the genes of Legok isolate were amplified with NP-1200f and NP-1529r pair and H5-131f and H5-580 pair primers. The result indicated that the Legok isolate was an avian influenza subtype H5N1. |
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| No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
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| Tag | Ind1 | Ind2 | Isi |
| 001 | INLIS000000000000078 | ||
| 005 | 20210323091254 | ||
| 008 | 210323||||||||| | ||| |||| || | | ||
| 020 | $a 0852-9612 | ||
| 035 | 0010-0321000078 | ||
| 041 | $a id | ||
| 100 | 0 | $a Enuh Raharjo Jusa | |
| 245 | 0 | 0 | $a Identifikasi Subtipe Virus Avian Influenza Isolat Legok Menggunakan Polymerase Chain Reaction |
| 250 | $a Vol. 10, p. 1-4 | ||
| 260 | $a Bogor $b BBPMSOH $c 2004 | ||
| 300 | $a 2 tables.:7 ref.: Summary (En) | ||
| 500 | $a The virology laboratory of Veterinary Drug Assay Laboratory isolated a virus which caused respiratory clinical signs in chicken from a layer farm, in Legok, Tangerang. The clinical signs of the disease were very similar to the avian influenza. The virus isolate was then identified by RT-PCR using primer NP-1200f and NP-1529r that amplified nucleoprotein gene of all influenza virus of poultry, man, pig and horse, and using 15 pair of H1-15 primer that amplified haemagglutinin antigen (HA) of avian subtype. The result of RT-PCR showed that both of the genes of Legok isolate were amplified with NP-1200f and NP-1529r pair and H5-131f and H5-580 pair primers. The result indicated that the Legok isolate was an avian influenza subtype H5N1. | ||
| 650 | 0 | $a PCR -- Electrophoresis -- Layer chickens -- ISOLATION -- JAVA -- Avian Influenza Virus -- Gene -- Agglutinins | |
| 700 | 0 | $a Bahruddin Syahroni Hasibuan | |
| 700 | 0 | $a Emilia | |
| 700 | 0 | $a Pudjiatmoko | |
| 700 | 0 | $a Sri Murni Astuti |
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