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Judul Detection Of Chlamydophila By Multiplex Polymerase Chain Reaction
Pengarang Pudjiatmoko
EDISI Vol. 9, p.7-16
Penerbitan Bogor BBPMSOH 2002
Deskripsi Fisik 6 tables.:26 ref.:Summary (En)
ISBN 0852-9612
Subjek PCR -- DNA -- ISOLATION -- CHLAMYDIA -- MICROORGANISMS -- RAPD
Catatan Molecular identification of 6 species of family chlamydiaceae carried out by nested polymerase chain reaction (PCR) with species-specific primers derived from 16s rRNA gene was investigated. The 16SA and 16SB primers were used for amplification of 1,473 bp 16S rRNA gene fragments of 25 strains of chlamydiaceae in the first PCR. A set of family-specific primer and 5 sets of species-specific primer were used for amplification of fragments of 16S rRNA gene in the nested PCR. The family-specific primers amplified all the 16S rRNA gene of chlamydophila spp. used The species-specific primers were shown to be specific for each species. Multiplex PCR carried out using 3 primers (2 sense primers and I antisense primers) in a single tube was applicable for detection of chlamydophila felis, chlamydophila caviae and chlamydophila psitaci. C. psittaci-specific primers could be used for detection of chlamydophila DNA extracted from 1 throat swab of human, 1 liver specimen of a parakeet and 2 fecal specimens of crows. C.fel
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No Barcode No. Panggil Akses Lokasi Ketersediaan
Tag Ind1 Ind2 Isi
001 INLIS000000000000079
005 20210323091255
008 210323||||||||| | ||| |||| || |
020 $a 0852-9612
035 0010-0321000079
041 $a en
100 0 $a Pudjiatmoko
245 0 0 $a Detection Of Chlamydophila By Multiplex Polymerase Chain Reaction
250 $a Vol. 9, p.7-16
260 $a Bogor $b BBPMSOH $c 2002
300 $a 6 tables.:26 ref.:Summary (En)
500 $a Molecular identification of 6 species of family chlamydiaceae carried out by nested polymerase chain reaction (PCR) with species-specific primers derived from 16s rRNA gene was investigated. The 16SA and 16SB primers were used for amplification of 1,473 bp 16S rRNA gene fragments of 25 strains of chlamydiaceae in the first PCR. A set of family-specific primer and 5 sets of species-specific primer were used for amplification of fragments of 16S rRNA gene in the nested PCR. The family-specific primers amplified all the 16S rRNA gene of chlamydophila spp. used The species-specific primers were shown to be specific for each species. Multiplex PCR carried out using 3 primers (2 sense primers and I antisense primers) in a single tube was applicable for detection of chlamydophila felis, chlamydophila caviae and chlamydophila psitaci. C. psittaci-specific primers could be used for detection of chlamydophila DNA extracted from 1 throat swab of human, 1 liver specimen of a parakeet and 2 fecal specimens of crows. C.felis-specific primers could be used for detection of chlamydophila DNA extracted from 4 throat swabs of humans and 2 conjunctival swabs of cats.
650 0 $a PCR -- DNA -- ISOLATION -- CHLAMYDIA -- MICROORGANISMS -- RAPD
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