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Tag
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Ind1
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Ind2
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Isi
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001
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INLIS000000000000081
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005
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20210323091255
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008
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210323||||||||| | ||| |||| || |
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020
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$a 0852-9612
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035
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0010-0321000081
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041
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$a id
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100
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0
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$a Pudjiatmoko
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245
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0
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0
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$a Respon pembentukan antibodi pada tikus putih terhadap vaksin ompA-Recombinant Feline Chlamydiosis
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250
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$a vol. 8, p.8-14
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260
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$a Bogor $b BBPMSOH $c 2001
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300
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$a 4 tables. 11 ref.; Summary (En)
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500
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$a The omp A-recombinant protein produced by E.coli BL21 (DE3)plysS containing pRSETA/ompA gene of feline Chlamydia psittaci B166 strain was purified using method of Xpress system protein purification (invitrogen BV, The Netherlands). To eliminate guanidine hydrochloride, the suspension of ompA-recombinant protein was dialyzed in 20 mM Tris pH 7.8, glycerol 10% and phenylmethylsulfonyl fluoride containing 0,1 M Mgcl2, CaCl2, NaCl or KCl. To prepare the vaccines, the purified proteins were emulsified with oil adjuvant. One ml of each vaccine was injected intramuscularly to 4 spesific pathogen free rats 8-week-old. The second vaccination was performed at 3 weeks post first vaccination. IgG antibody titer against feline Chlamydia psittaci was measured using microimmunofluorescene test at a week post second vaccination. Vaccination using ompA-recombinant could initiate IgG antibody in 21 of 24 vaccinated rats. IgG antibody titer or rats vaccinated with materials dialyzed in dialysis buffer containing KCl has highest titer ranged from 1:16 to 1:64, and its geometric mean titer is 1:38. The results of the present study indicate that the ompA-recombinant protein of chlamydia used for main component of vaccine my initiate antibody against feline C. psittaci in rats
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650
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0
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$a Cats -- Escherichia coli -- Rats -- ANTIBODIES -- IMMUNO FLUORESCENCE -- Chlamydia Psittaci -- Bacteriosis -- Synthetic Vaccine -- Protein -- Specific Pathogen Free State
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