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Judul Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle / Saepulloh, Muharam; Sendow, Indrawati (Indonesian Research Center for Veterinary Science)
Pengarang Sendow, Indrawati (Indonesian Research Center for Veterinary Science)
EDISI Jurnal Veteriner
Penerbitan 2015
Deskripsi Fisik Vol.16 (1), p.1-7
Subjek BVDV, RT-PCR, genotype-1.
Abstrak Bovine viral diarrhea virus (BVDV) is an important viral disease, which a ubiquitous pathogen of cattle with worldwide economic importance and due to its misdiagnose with other viruses. The goal of the current study was to identify and characterize of BVDV by reverse transcriptase polymerase chain reaction (RT-PCR) and followed by sequence genome analyses. Blood, feces, and semen samples were collected from 588 selected cattle from animals suffering from diarrhea and respiratory manifestation. RT- PCR results showed that the 69 (11.74%) samples were positive to BVDV. Further molecular characterization was conducted only with 17 PCR positive samples. The results indicated the 17 Indonesian BVD virus isolates were belonging to the genotype-1 of BVDV (BVDV-1) based on sequence analysis and a phylogenetic relationship between Indonesian BVDV isolates and BVDV in the world. This finding is the first report of BVD-1 circulated in Indonesian cattle
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No Barcode No. Panggil Akses Lokasi Ketersediaan
ARTVET2068 ARTVET2068 Dapat dipinjam Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Koleksi Agris Tersedia
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035 # # $a 0010-0721002254
082 # # $a ARTVET2068
084 # # $a ARTVET2068
245 # # $a Identification and Characterization of Bovine Viral Diarrhea Virus from Indonesian Cattle /$c Saepulloh, Muharam; Sendow, Indrawati (Indonesian Research Center for Veterinary Science)
250 # # $a Jurnal Veteriner
260 # # ,$c 2015
300 # # $a Vol.16 (1), p.1-7
520 # # $a Bovine viral diarrhea virus (BVDV) is an important viral disease, which a ubiquitous pathogen of cattle with worldwide economic importance and due to its misdiagnose with other viruses. The goal of the current study was to identify and characterize of BVDV by reverse transcriptase polymerase chain reaction (RT-PCR) and followed by sequence genome analyses. Blood, feces, and semen samples were collected from 588 selected cattle from animals suffering from diarrhea and respiratory manifestation. RT- PCR results showed that the 69 (11.74%) samples were positive to BVDV. Further molecular characterization was conducted only with 17 PCR positive samples. The results indicated the 17 Indonesian BVD virus isolates were belonging to the genotype-1 of BVDV (BVDV-1) based on sequence analysis and a phylogenetic relationship between Indonesian BVDV isolates and BVDV in the world. This finding is the first report of BVD-1 circulated in Indonesian cattle
650 # 4 $a BVDV, RT-PCR, genotype-1.
700 # $a Sendow, Indrawati (Indonesian Research Center for Veterinary Science)
990 # # $a ARTVET2068
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