| Judul | Application of Elisa to Detect Bluetongue Virus Group Infection / Sendow, Indrawati |
| Pengarang | Sendow, Indrawati Daniels, Peter |
| EDISI | Penyakit Hewan, Vol. 25 (46 A), p. 68-71. |
| Penerbitan | 1993 |
| Deskripsi Fisik | 4 |
| Subjek | Elisa, bluetongue virus |
| Abstrak | A competitive enzyme-Jinked immunosorbent assay (C-ELISA) using a group specific monoclonal antibody (MAb) against the bluetongue viruses (B'IV) was established to detect antibodies against B1V. Sera from sentinel cattle in West Java were tested. The results of the C-ELISA were compared with the classical method for detection of antibodies to the bluetongue group, the agar gel immunodiffusion test. Increased specificity was observed using the Mab in the B1V C-ELISA. Not all sera that reacted in the AGID test reacted in the C-ELISA, and this was interpreted as the elimination of cross-reactions to other orbiviruses, including epizootic haemorrhagic disease of deer viruses, that are a problem in the agar gel immunodiffusioa (AGID) test. Increased sensitivity with this test was also observed as seroconversions to B1V were detected earlier by C-EUSA than with theAGID. The CELISA not only has increased sensitivity and speci~city, but also cost less, and can be used for sera from all species, and should re |
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| No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
|---|---|---|---|---|
| ARTVET2050 | ARTVET2050 | Dapat dipinjam | Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Koleksi Agris | Tersedia |
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| 001 | INLIS000000000017704 | ||
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| 082 | # | # | $a ARTVET2050 |
| 084 | # | # | $a ARTVET2050 |
| 100 | 0 | # | $a Sendow, Indrawati |
| 245 | 1 | # | $a Application of Elisa to Detect Bluetongue Virus Group Infection /$c Sendow, Indrawati |
| 250 | # | # | $a Penyakit Hewan, Vol. 25 (46 A), p. 68-71. |
| 260 | # | # | ,$c 1993 |
| 300 | # | # | $a 4 |
| 520 | # | # | $a A competitive enzyme-Jinked immunosorbent assay (C-ELISA) using a group specific monoclonal antibody (MAb) against the bluetongue viruses (B'IV) was established to detect antibodies against B1V. Sera from sentinel cattle in West Java were tested. The results of the C-ELISA were compared with the classical method for detection of antibodies to the bluetongue group, the agar gel immunodiffusion test. Increased specificity was observed using the Mab in the B1V C-ELISA. Not all sera that reacted in the AGID test reacted in the C-ELISA, and this was interpreted as the elimination of cross-reactions to other orbiviruses, including epizootic haemorrhagic disease of deer viruses, that are a problem in the agar gel immunodiffusioa (AGID) test. Increased sensitivity with this test was also observed as seroconversions to B1V were detected earlier by C-EUSA than with theAGID. The CELISA not only has increased sensitivity and speci~city, but also cost less, and can be used for sera from all species, and should replace the AGID test for bluetongue serology |
| 650 | # | 4 | $a Elisa, bluetongue virus |
| 700 | # | $a Daniels, Peter | |
| 990 | # | # | $a ARTVET2050 |
| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | ARTVET2050.pdf | ARTVET2050 | Baca Online |
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