01786     2200205   4500001002100000005001500021035002000036007000300056008003900059100003200098245006200130260010300192300000900295650002300304700002100327700001900348520117300367600002401540600001601564INLIS00000000000216620221220111657  a0010-1222000010ta221220                |          | |  0 aRasa, Fadjar Sumping Tjatur1 aThe Hemolytic Activity of Bracken Extracts in Guinea Pigs  aMinami :bDepartment of Veterinary Pharmacology, Faculty of Agriculture, Tottori University,c1998  a5 p. 4aHAEMOLYTIC ANAEMIA0 aSaito, Toshiyuki0 aSatoh, Hiroshi  aThis study was conducted to elucidate the hemolytic activity of a new toxic substance in bracken fern. A crude extract (CE)
was prepared from the methanol extracts of bracken by the column chromatography. When the CE was injected subcutaneously in guinea pigs, the hemoglobinuria and hemolysis were observed within 6 hr, and 3 days later edema and hemorrhages in the urinary bladder were observed. The CE was then fractionated by high performance liquid chromatography (HPLC), and three (HF, BF and CF) of the fractions showed the toxic activities in guinea pigs. The HF caused the hemolysis, whereas both the BF and the CF caused the hemorrhagic cystitis without any hemolytic activities. The HF was further fractionated by the HPLC, resulting of the 3 fractions (HF-I, II and III). The hemolysis was caused only with the HF-II, and HF-II as well as HF did not cause the hemorrhagic cystitis. HPLC analysis revealed that both BF and CF contains braxin B and braxin C, respectively, and both HF and HF-II do not contain braxin A, B or C. These facts suggest that bracken fern contains a new toxic substance (hemolysin) which induces the acute hemolysis in guinea pigs 4aPTERIDIUM AQUILINUM 4aGUINEA PIGS