na INLIS000000000019325 20250227025250 0010-0225000011 ta 250227 | | | ARTVET2505 ARTVET2505 AJE d Ajeng Fabeane Putri Detection of Very Virulent Infectious Bursal Disease (IBD) in Chicken in West Java / Ajeng Fabeane Putri Vol. 18 (1): 37-43, Februari 2024 Banda Aceh, Indonesia : Fakulitas Kedokteran Hewan Universitas Syiah Kuala, 2024 7 : ill IBD REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ISOLATION CHICKEN Agus Winarsongko Heri Hoerudin, Gita Sekarmila Ahpan Jejen Jaelani Wawan Gunawan Rina Dewiyanti Yuda Pratama Harimurti Nuradji Nuha Fairusya Fitrine Ekawasti Rahmat Setya Adji NLP Indi Dharmayanti Risa Indriani Bambang Ngaji Utomo Sri Suryatmiati Infectious Bursal Disease (IBD), also known as Gumboro disease, is an acute, highly contagious disease that infects chickens and causes a high mortality rate of up to 100% in young animals. The disease is caused by Infectious Bursal Disease Virus (IBDV) of the genus Avibirnavirus, family Birnaviridae. The disease has been reported in Indonesia since 1976, and management strategies for the disease, such as vaccination, have been applied to prevent and control outbreaks in poultry farms. In this study, we conducted the detection of the disease in chickens from a farm in West Java with a mortality rate of 80%. Chickens showing clinical signs, such as sudden death, anorexia, watery diarrhea, and ruffled feathers, were necropsied, and organ samples, including the bursa Fabricius, brain, and spleen, were collected. The samples were then tested using Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) to confirm the diagnosis of IBD. Positive results were obtained in this study, highlighting the need for improved biosecurity in poultry farms in Indonesia. These results also provided a basis for further research on viral characterization to develop detection kits or vaccines for IBD using local isolates from the field in Indonesia. INDONESA DOI: https://doi.org/10.21157/j.med.vet..v14i2.35819 ARTVET2505