na INLIS000000000018758 20240212082133 0010-0224000008 ta 240212 | | | ARTVET2384 ARTVET2384 Blacksell, Stuart D. The Biosafety Research Road Map : The Search for Evidence to Support Practices in the Laboratory—Shigella spp. / Blacksell, Stuart D. Applied Biosafety, Vol, 28, No, 2. 2023 96–101 Shigella spp pathogen characteristics biosafety evidence knowledge gap analysis disinfection inactivation Dhawan, Sandhya Kusumoto, Marina Le, Kim Khanh Davis, Ben J. Summermatter, Kathrin O’Keefe, Joseph Kozlovac, Joseph Almuhairi, Salama Suhail Sendow, Indrawati Scheel, Christina M. Ahumibe, Anthony Masuku, Zibusiso M. Bennett, Allan M. Kojima, Kazunobu Harper, David R. Hamilton, Keith Introduction: Shigella bacteria cause shigellosis, a gastrointestinal infection most often acquired from con taminated food or water. Methods: In this review, the general characteristics of Shigella bacteria are described, cases of laboratory acquired infections (LAIs) are discussed, and evidence gaps in current biosafety practices are identi?ed. Results: LAIs are undoubtedly under-reported. Owing to the low infectious dose, rigorous biosafety level 2 practices are required to prevent LAIs resulting from sample manipulation or contact with infected sur faces. Conclusions: It is recommended that, before laboratory work with Shigella, an evidence-based risk assess ment be conducted. Particular emphasis should be placed on personal protective equipment, handwash ing, and containment practices for procedures that generate aerosols or droplets. DOI: 10.1089/apb.2022.0046