na INLIS000000000018756 20250203105722 0010-0224000006 ta 250203 | | | ARTVET2353 ARTVET2353 Blacksell, Stuart D. The Biosafety Research Road Map : The Search for Evidence to Support Practices in the Laboratory—Bacillus anthracis and Brucella melitensis / Blacksell, Stuart D. Applied Biosafety, Vol, 28, No, 2. 2023 72–86 Bacillus anthracis Brucella melitensis pathogen characteristics biosafety evidence biosafety knowledge gap Dhawan, Sandhya Kusumoto, Marina Le, Kim Khanh Summermatter, Kathrin O’Keefe, Joseph Kozlovac, Joseph Almuhairi, Salama Suhail Sendow, Indrawati M Christina Scheel Ahumibe, Anthony Masuku, Zibusiso M. Bennett, Allan M. Kojima, Kazunobu Harper, David R. Hamilton, Keith Introduction: Brucella melitensis and Bacillus anthracis are zoonoses transmitted from animals and animal products. Scienti?c information is provided in this article to support biosafety precautions necessary to protect laboratory workers and individuals who are potentially exposed to these pathogens in the workplace or other settings, and gaps in information are also reported. There is a lack of information on the appropriate effective concentration for many chemical disinfectants for this agent. Controversies related to B. anthracis include infectious dose for skin and gastrointestinal infections, proper use of personal protective equipment (PPE) during the slaughter of infected animals, and handling of contaminated materials. B. melitensis is reported to have the highest number of laboratory-acquired infections (LAIs) to date in laboratory workers. Methods: A literature search was conducted to identify potential gaps in biosafety and focused on ?ve main sections including the route of inoculation/modes of transmission, infectious dose, LAIs, containment releases, and disinfection and decontamination strategies. Results: Scienti?c literature currently lacks information on the effective concentration of many chemical dis- infectants for this agent and in the variety of matrices where it may be found. Controversies related to DOI: 10.1089/apb.2022.0042