na INLIS000000000018075 20250203105054 0010-0222000049 ta 250203 | | | ARTVET2343 Wardhana, April Hari Occurrence and genetic identifications of porcine Entamoeba / Wardhana, April Hari Parasitology Research 2020 1-16 halaman Entamoeba polecki Entamoeba suis Gastrointestinal parasites Subtype Sawitri, Dyah Haryuningtyas Ekawasti, Fitrine Martindah, Eny Apritadewi, Dias Shibahara, Tomoyuki Kusumoto, Masahiro Tokoro, Masaharu Sasai, Kazumi Matsubayashi, Makoto Entamoeba suis and E. polecki subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196 fecal samples of pigs (118 of adults, 78 of piglets) at Tangerang in West Java, Indonesia, in 2017, employing PCR using porcine Entamoeba-specific primers. E. suis was the more frequently detected species, observed in 81.1% of samples, while E. polecki ST1 and ST3 were detected in 18.4% and 17.3% of samples, respectively; mixed infections (harboring 2–3 species or subtypes of Entamoeba) were confirmed in 29.3% of positive samples. Statistically significant differences in the positive rates were not seen between adult pigs and piglets, except for those of E. polecki ST3. The prevalences of Eimeria spp. and/or Cystoisospora suis (79.1%), strongyles (55.6%), and Strongyloides spp. (6.1%) were also observed morphologically in the samples. Further chronological or seasonal investigations of pigs and humans in these high-prevalence areas are needed to assess the virulence of the Entamoeba parasites, including the effects on pig productivity, and to evaluate the zoonotic impacts of these organisms Indonesia https://doi.org/10.1007/s00436-020-06806-0 ARTVET2343 ARTVET2343