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Judul Restriction fragment length polymorphism analysis of genes of virulent strain isolate of Toxoplasma gondii using enzyme DdeI / Fitrine Ekawasti1,2
Pengarang Umi Cahyaningsih2
N. L. P. Indi Dharmayanti1
Siti Sa’diah3
Didik Tulus Subekti1
Zul Azmi1
Muhammad Ibrahim Desem1
EDISI Vol.7/No.2/7
Penerbitan 1. Indonesian Research Center for Veterinary Sciences : International Journal of One Health, 2021
Deskripsi Fisik 8
Subjek Restriction fragment length
Abstrak oxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii. The pathogenicity of this species depends on the type originating from the clonal population. Techniques for more accurately determining the type of T. gondii have recently been developed using genetic markers. Specifically, T. gondii has been typed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). This study aimed to identify sets of PCR-RFLP markers that have high power to discriminate genotyping of T. gondii and are easy to use and are easy to use. The objective of this study was to characterize virulent strain isolates of T. gondii by PCR-RFLP using 10 markers with DdeI.
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No Barcode No. Panggil Akses Lokasi Ketersediaan
00000033215 ARTVET2392 Dapat dipinjam Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Ruang Baca Umum Tersedia
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082 # # $a ARTVET2392
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245 # # $a Restriction fragment length polymorphism analysis of genes of virulent strain isolate of Toxoplasma gondii using enzyme DdeI /$c Fitrine Ekawasti1,2
250 # # $a Vol.7/No.2/7
260 # # $a 1. Indonesian Research Center for Veterinary Sciences :$b International Journal of One Health,$c 2021
300 # # $a 8
520 # # $a oxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii. The pathogenicity of this species depends on the type originating from the clonal population. Techniques for more accurately determining the type of T. gondii have recently been developed using genetic markers. Specifically, T. gondii has been typed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). This study aimed to identify sets of PCR-RFLP markers that have high power to discriminate genotyping of T. gondii and are easy to use and are easy to use. The objective of this study was to characterize virulent strain isolates of T. gondii by PCR-RFLP using 10 markers with DdeI.
650 # 4 $a Restriction fragment length
700 0 # $a Didik Tulus Subekti1
700 0 # $a Muhammad Ibrahim Desem1
700 0 # $a N. L. P. Indi Dharmayanti1
700 0 # $a Siti Sa’diah3
700 0 # $a Umi Cahyaningsih2
700 0 # $a Zul Azmi1
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