| Judul | EVALUASI MENGENAI SPESIFISTTAS ANTIBODI MONOKLONAL K99 TERHADAP ANTIGEN PERLEKATAN ESCHERICHIA COLI ENTEROTOKSIGENIK / GOZALI R. MOEKTI |
| Pengarang | GOZALI R. MOEKTI SUPAR KUSMIYATI |
| Penerbitan | Balai Penelitian Veteriner, Bogor : Prosiding Seminar Teknologi Veteriner 7h., 1994 |
| Deskripsi Fisik | 12 |
| Subjek | ENGENAI SPESIFISTTAS ANTIBODI |
| Abstrak | In attempt to develop an improved diagnostic tool for neonatal colibacillosis in calves, monoclonal antibodies (McAb) directed against K99-adhesin derived from a reference strain of enterotoxigenic Escheridda con (Compton, K12; K99) were produced. Specificity of McAb produced was evaluated using a slide conglutination teat, indirect enzyme-linked immunosothent assay (ELISA), and immunoblotting. Antigens used for the specificity testing were prepared from known E. coif K99 of bath homologous (Compton, KU; K99) and heterologous (841), field-isolates (BL413, G1231, G1150 and 2631), and those of known as 1(99-negative E. coil (i.e. types 1(88, F41 and 987P). The present study resulted in a panel of 20 hybridomas secreting antibodies specific to K99-adhesin. Two of the total hybridomas obtained, 11(99-B5 and 1K99-C9, were cloned consecutively producing 14 and 10 clones, which were then found to be stabile up to the second cloning. Of 24 McAbs produced from the total clones, three antibodies were tested usi |
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| No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
|---|---|---|---|---|
| ARTVET0761 | ARTVET0761 | Dapat dipinjam | Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Ruang Baca Umum | Tersedia |
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| 100 | 0 | # | $a GOZALI R. MOEKTI |
| 245 | 1 | # | $a EVALUASI MENGENAI SPESIFISTTAS ANTIBODI MONOKLONAL K99 TERHADAP ANTIGEN PERLEKATAN ESCHERICHIA COLI ENTEROTOKSIGENIK /$c GOZALI R. MOEKTI |
| 260 | # | # | $a Balai Penelitian Veteriner, Bogor :$b Prosiding Seminar Teknologi Veteriner 7h.,$c 1994 |
| 300 | # | # | $a 12 |
| 520 | # | # | $a In attempt to develop an improved diagnostic tool for neonatal colibacillosis in calves, monoclonal antibodies (McAb) directed against K99-adhesin derived from a reference strain of enterotoxigenic Escheridda con (Compton, K12; K99) were produced. Specificity of McAb produced was evaluated using a slide conglutination teat, indirect enzyme-linked immunosothent assay (ELISA), and immunoblotting. Antigens used for the specificity testing were prepared from known E. coif K99 of bath homologous (Compton, KU; K99) and heterologous (841), field-isolates (BL413, G1231, G1150 and 2631), and those of known as 1(99-negative E. coil (i.e. types 1(88, F41 and 987P). The present study resulted in a panel of 20 hybridomas secreting antibodies specific to K99-adhesin. Two of the total hybridomas obtained, 11(99-B5 and 1K99-C9, were cloned consecutively producing 14 and 10 clones, which were then found to be stabile up to the second cloning. Of 24 McAbs produced from the total clones, three antibodies were tested using a slide agglutination test and proved to be reactive to K99 antigens prepared either from reference strains or field-isolates, but showed not to be reactive to 1(99-negative E. ca. Based on the indirect-ELISA, all McAbs produced so far were indicated to be only recognise 1(99 antigens. Moreover immunolgotting utukttaken in this study demonstrated that the 24 McAbs identify 1(99 antigen located at a molecular weight marker ranging from 16000 to 17,000 &ikons (D). |
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| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | 761.pdf | ARTVET761 | Baca Online |
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