| Judul | KLONING MOLEKUL DNA GENOMIK ESCHERICHIA COL' ENTEROTOKSIGENIK 98W : SEBUAH KAJIAN MENGENAI PENGEMBANGAN VAKSIN SECARA REKAYASA GENETIKA / KUSMIYATI |
| Pengarang | KUSMIYATI SUPAR G.R. MOEKTI |
| Penerbitan | Balai Penelitian Veteriner, Bogor : Prosiding Seminar Teknologi Veteriner 7h., 1994 |
| Deskripsi Fisik | 11 |
| Subjek | MOLEKUL DNA GENOMIK |
| Abstrak | In order to analyse DNA fragments encoding synthesis of adhesin antigens in enterotoxigenic Escherichia cell 987P, a blind molecular cloning was undertaken. Genomic DNA was extracted from field isolates of E. coil bearing 987P adhesin, and fragmented utilizing restriction enzymes Sau3A or EcoRV. The fragmented genomic DNA was then used to generate libraries in the E. coil XL1-Blue expression system employing phagemid pBluescript II SK vector. AU clones suspected recombinant DNA were grown on selective medium for propagating 987P adhesin antigen. Six recombinat clones (mutants) were selected to be tested serologically using antiserum anti 987P adhesin antigen raised in rabbits. Of the 15 mutants, 13 of which were found to be able to express a homologous polypeptide compound against 987P anti adhesin antibody. The present study may therefore suggest that a DNA fragment clone is located in the area of coding sequence for the synthesis of adhesin antigen in enterotoxigenic Escherichia cob 987P. Further st |
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| Target Pembaca | Tidak ada kode yang sesuai |
| No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
|---|---|---|---|---|
| 00000033000 | ARTVET759 | Dapat dipinjam | Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Ruang Baca Umum | Tersedia |
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| 100 | 0 | # | $a KUSMIYATI |
| 245 | 1 | # | $a KLONING MOLEKUL DNA GENOMIK ESCHERICHIA COL' ENTEROTOKSIGENIK 98W : $b SEBUAH KAJIAN MENGENAI PENGEMBANGAN VAKSIN SECARA REKAYASA GENETIKA /$c KUSMIYATI |
| 260 | # | # | $a Balai Penelitian Veteriner, Bogor :$b Prosiding Seminar Teknologi Veteriner 7h.,$c 1994 |
| 300 | # | # | $a 11 |
| 520 | # | # | $a In order to analyse DNA fragments encoding synthesis of adhesin antigens in enterotoxigenic Escherichia cell 987P, a blind molecular cloning was undertaken. Genomic DNA was extracted from field isolates of E. coil bearing 987P adhesin, and fragmented utilizing restriction enzymes Sau3A or EcoRV. The fragmented genomic DNA was then used to generate libraries in the E. coil XL1-Blue expression system employing phagemid pBluescript II SK vector. AU clones suspected recombinant DNA were grown on selective medium for propagating 987P adhesin antigen. Six recombinat clones (mutants) were selected to be tested serologically using antiserum anti 987P adhesin antigen raised in rabbits. Of the 15 mutants, 13 of which were found to be able to express a homologous polypeptide compound against 987P anti adhesin antibody. The present study may therefore suggest that a DNA fragment clone is located in the area of coding sequence for the synthesis of adhesin antigen in enterotoxigenic Escherichia cob 987P. Further studies may be required particularly in connection to the determination of the sequence of DNA fragment clone, expression of gene clone, and the potential use of expressing components as sub-unit vaccines. |
| 650 | # | 4 | $a MOLEKUL DNA GENOMIK |
| 700 | 0 | # | $a G.R. MOEKTI |
| 700 | 0 | # | $a SUPAR |
| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | 759.pdf | ARTVET759 | Baca Online |
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