| Judul | Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Antibody in Chickens Using Local Isolate of PTS III / Indriani, Risa ; Dharmayanti, NLP Indi (Balai Besar Penelitian Veteriner) |
| Pengarang | Dharmayanti, NLP Indi Indriani, Risa |
| EDISI | Jurnal Biologi Indonesia |
| Penerbitan | 2013 |
| Deskripsi Fisik | Vol. 9 (2), p.159-166. |
| Subjek | ELISA, antibody, chicken, IB PTS-III local isolate |
| Abstrak | An indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis (IBV). Antigen was prepared from whole virus of infectious bronchitis local isolate PTS-III serotype. Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA were determined 0.4pg/well, 1:2000 and 1:100, respectively. Correlation optical densities (OD) were compared with a standard commercial ELISA (R2=0.933). The developed ELISA has a better sensitivity to hemagglutination inhibition (HI) test. The developed local isolate ELISA can be used to detect antibody against infectious bronchitis virus and it is suitable for sample screening at the diagnostic laboratories. |
| Bentuk Karya | Tidak ada kode yang sesuai |
| Target Pembaca | Tidak ada kode yang sesuai |
| No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
|---|---|---|---|---|
| ARTVET2301 | ARTVET2301 | Dapat dipinjam | Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Koleksi Agris | Tersedia |
| Tag | Ind1 | Ind2 | Isi |
| 001 | INLIS000000000017794 | ||
| 005 | 20210806094518 | ||
| 008 | 210806################|##########|#|## | ||
| 035 | # | # | $a 0010-0721002326 |
| 082 | # | # | $a ARTVET2301 |
| 084 | # | # | $a ARTVET2301 |
| 100 | 1 | # | $a Indriani, Risa |
| 245 | 1 | # | $a Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Antibody in Chickens Using Local Isolate of PTS III /$c Indriani, Risa ; Dharmayanti, NLP Indi (Balai Besar Penelitian Veteriner) |
| 250 | # | # | $a Jurnal Biologi Indonesia |
| 260 | # | # | ,$c 2013 |
| 300 | # | # | $a Vol. 9 (2), p.159-166. |
| 520 | # | # | $a An indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis (IBV). Antigen was prepared from whole virus of infectious bronchitis local isolate PTS-III serotype. Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA were determined 0.4pg/well, 1:2000 and 1:100, respectively. Correlation optical densities (OD) were compared with a standard commercial ELISA (R2=0.933). The developed ELISA has a better sensitivity to hemagglutination inhibition (HI) test. The developed local isolate ELISA can be used to detect antibody against infectious bronchitis virus and it is suitable for sample screening at the diagnostic laboratories. |
| 650 | # | 4 | $a ELISA, antibody, chicken, IB PTS-III local isolate |
| 700 | 1 | # | $a Dharmayanti, NLP Indi |
| 990 | # | # | $a ARTVET2301 |
| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | ARTVET2301.pdf | ARTVET2301 | Baca Online |
Content Unduh katalog
Karya Terkait :