| Judul | Bacterial expression of larval peritrophins of Chryosomya bezziana. / Wijffels, Gene; Vuocolo, Tony; Muharsini, Sri[Research Institute for Veterinary Science]Supriyanti, Florentina. |
| Pengarang | Vuocolo, Tony Muharsini, Sri[Research Institute for Veterinary Science]Supriyanti, Florentina. |
| EDISI | Jurnal Ilmu Ternak dan Veteriner. |
| Penerbitan | 2000 |
| Deskripsi Fisik | Vol.5 (3): p.170-176. 2000. |
| Subjek | Chrysomya, peritrophic membrane, peritrophin, recombinant protein, hexaHis tag, GST |
| Abstrak | Three candidate antigens, Chrysomya bezziana peritrophin-48, Chrysomya bezziana peritrophin-15 and Chrysomya bezziana peritrophin-42, were prepared for recombinant protein production in Escherichia coli using a variety of expression vectors. Cb peritrophin-48 was expressed as a recombinant protein possessing a carboxy-terminal hexaHis tag. Cb peritrophin-15 was expressed as both a glutathione S-transferase fusion protein and as an amino-terminal hexaHis tagged protein. The glutathione Stransferase Cb peritrophin-15 construct produced a heterogeneous group of fusion proteins. Cb peritrophin-42 was also expressed as an amino-terminal hexaHis tagged protein. The two putative domains of Cb peritrophin-42 were also separately expressed, again with amino-terminal hexaHis tags. Cultures of the hexaHis constructs Cb peritrophin-48, -15 and —42 were demonstrated to be useful for the production and purification of these protein antigens and were scaled-up for vaccine trials and protein characterization st |
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| No Barcode | No. Panggil | Akses | Lokasi | Ketersediaan |
|---|---|---|---|---|
| ARTVET2043 | ARTVET2043 | Dapat dipinjam | Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Koleksi Agris | Tersedia |
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| 001 | INLIS000000000017697 | ||
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| 245 | # | # | $a Bacterial expression of larval peritrophins of Chryosomya bezziana. /$c Wijffels, Gene; Vuocolo, Tony; Muharsini, Sri[Research Institute for Veterinary Science]Supriyanti, Florentina. |
| 250 | # | # | $a Jurnal Ilmu Ternak dan Veteriner. |
| 260 | # | # | ,$c 2000 |
| 300 | # | # | $a Vol.5 (3): p.170-176. 2000. |
| 520 | # | # | $a Three candidate antigens, Chrysomya bezziana peritrophin-48, Chrysomya bezziana peritrophin-15 and Chrysomya bezziana peritrophin-42, were prepared for recombinant protein production in Escherichia coli using a variety of expression vectors. Cb peritrophin-48 was expressed as a recombinant protein possessing a carboxy-terminal hexaHis tag. Cb peritrophin-15 was expressed as both a glutathione S-transferase fusion protein and as an amino-terminal hexaHis tagged protein. The glutathione Stransferase Cb peritrophin-15 construct produced a heterogeneous group of fusion proteins. Cb peritrophin-42 was also expressed as an amino-terminal hexaHis tagged protein. The two putative domains of Cb peritrophin-42 were also separately expressed, again with amino-terminal hexaHis tags. Cultures of the hexaHis constructs Cb peritrophin-48, -15 and —42 were demonstrated to be useful for the production and purification of these protein antigens and were scaled-up for vaccine trials and protein characterization studies. |
| 650 | # | 4 | $a Chrysomya, peritrophic membrane, peritrophin, recombinant protein, hexaHis tag, GST |
| 700 | # | $a Muharsini, Sri[Research Institute for Veterinary Science]Supriyanti, Florentina. | |
| 700 | # | $a Vuocolo, Tony | |
| 990 | # | # | $a ARTVET2043 |
| No | Nama File | Nama File Format Flash | Format File | Action |
| 1 | ARTVET2043.pdf | ARTVET2043 | Baca Online |
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