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Judul Chicken recombinant antibodies specific for very virulent infectious bursal disease virus. / Parede, Lies(Balai Penelitian Veteriner, Bogor Indonesia)S. I. Sapats; L. Trinidad; G. Gould; H. G. Heine; J. Ignjatovic(CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia)T. P. van den Berg(Avian Virology Immunology Vet
Pengarang L. Trinidad
G. Gould
H. G. Heine
J. Ignjatovic(CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia)T. P. van den Berg(Avian Virology Immunology Vet
Parede, Lies
EDISI Archives of Virology
Penerbitan 2006
Deskripsi Fisik Vol. 151 p. 1551-1566. (2006)
Subjek infectious bursal disease virus
Abstrak A phage-displayed single chain variable fragment (scFv) antibody library was constructed from the immune spleen cells of chickens immunized with very virulent infectious bursal disease virus (vvIBDV) strain CS89. A library consisting of around 9.2×107 clones was subjected to 3 rounds of panning against captured CS89 virus. Analysis of individual clones by nucleotide sequencing revealed at least 22 unique scFv antibodies binding to vvIBDV in ELISA. Testing of the scFv antibody panel in ELISA against classical, variant or vaccine strains and a wide variety of vvIBDV isolates from the UK, China, France, Belgium, Africa, Brazil, Indonesia and the Netherlands identified one antibody, termed chicken recombinant antibody 88 (CRAb 88) that was specific for vvIBDV. CRAb 88 was capable of recognizing all vvIBDV strains tested regardless of their country of origin and showed no reactivity with classical, variant or vaccine strains, lending support to the use of this scFv as a powerful diagnostic tool for the
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No Barcode No. Panggil Akses Lokasi Ketersediaan
ARTVET1449 ARTVET1449 Dapat dipinjam Perpustakaan Balai Besar Pengujian Standar Instrumen Veteriner - Koleksi Agris Tersedia
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100 0 # $a Parede, Lies
245 1 # $a Chicken recombinant antibodies specific for very virulent infectious bursal disease virus. /$c Parede, Lies(Balai Penelitian Veteriner, Bogor Indonesia)S. I. Sapats; L. Trinidad; G. Gould; H. G. Heine; J. Ignjatovic(CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia)T. P. van den Berg(Avian Virology Immunology Vet
250 # # $a Archives of Virology
260 # # ,$c 2006
300 # # $a Vol. 151 p. 1551-1566. (2006)
520 # # $a A phage-displayed single chain variable fragment (scFv) antibody library was constructed from the immune spleen cells of chickens immunized with very virulent infectious bursal disease virus (vvIBDV) strain CS89. A library consisting of around 9.2×107 clones was subjected to 3 rounds of panning against captured CS89 virus. Analysis of individual clones by nucleotide sequencing revealed at least 22 unique scFv antibodies binding to vvIBDV in ELISA. Testing of the scFv antibody panel in ELISA against classical, variant or vaccine strains and a wide variety of vvIBDV isolates from the UK, China, France, Belgium, Africa, Brazil, Indonesia and the Netherlands identified one antibody, termed chicken recombinant antibody 88 (CRAb 88) that was specific for vvIBDV. CRAb 88 was capable of recognizing all vvIBDV strains tested regardless of their country of origin and showed no reactivity with classical, variant or vaccine strains, lending support to the use of this scFv as a powerful diagnostic tool for the differentiation of vvIBDV strains. Immunoprecipitation studies revealed that CRAb 88 was directed towards a highly conformational epitope located within the major neutralizing protein VP2. Sequence analysis of the hypervariable region of VP2 of the IBDV strains tested indicate that Ile(256) and Ile(294) may play roles in binding of CRAb 88. This is the first reagent of its type capable of positively distinguishing vvIBDV from other IBDV strains.
650 # 4 $a infectious bursal disease virus
700 # $a G. Gould
700 # $a H. G. Heine
700 # $a J. Ignjatovic(CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia)T. P. van den Berg(Avian Virology Immunology Vet
700 # $a L. Trinidad
990 # # $a ARTVET1449
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