02100     2200301   4500001002100000005001500021035002000036007000300056008003900059082001500098084002100113100001700134245012500151250006600276260006000342300001300402650001000415650001800425650001100443700001900454700001700473700001000490700001900500520122600519600001001745856002801755990001501783INLIS00000000001933820250310013008  a0010-0225000024ta250310                |          | |    aARTVET2511  aARTVET2511 SUM d0 aSumarningsih1 aDevelopment of In-House ELISA using recombinant LipL32 for Detection of Human Leptospirosis in Indonesia /cSumarningsih  aJurnal Sain Veteriner, Vol. 42. No. 1. April 2024, Hal. 32-36  aYogyakarta, Indonesia :bUniversitas Gadjah Mada,c2024  a5 :bill 4aHUMAN 4aLEPTOSPIROSIS 4aLIPL320 aGita Sekarmila0 aAndi Mulyadi0 aAhpas0 aSimson Tarigan  aEarly laboratory confirmation is important for the accurate diagnosis and treatment of patient infected by leptospirosis. Microscopic agglutination test (MAT) as the gold standard for detection of human leptospirosis has many limitation and only available in reference laboratories. Therefore, many studies suggested LipL32 protein as a good candidate for development of leptospirosis detection kit because it is highly conserved and produced only in pathogenic Leptospira species. In this study, we aim to investigate the performance of our in-house ELISA using recombinant LipL32 to detect leptospirosis in Indonesia. Fourteen human sera were used in this study and the infection status were determine using MAT. The result showed that nine of eleven MAT positive sera were successfully recognized by LipL32 ELISA. The antibody binding to LipL32 was also confirm by immunoblot. There was one of three MAT negative sera has high OD above 0.5 in ELISA, but it showed negative reaction in immunoblot result. Overall, this study demonstrated that recombinant LipL32 protein can recognized antibody from human leptospirosis and can be used as a universal antigen to detect infection by any serovars of pathogenic leptospira. 4aELISA  aDOI: 10.22146/jsv.90085  aARTVET2511