03085     2200409   4500001002100000005001500021035002000036007000300056008003900059082001500098100003300113245015200146260005300298300001400351700001500365700001000380700002400390700001500414700001500429700001900444700001300463700001900476700001800495520181000513856004002323250007302363650004302436650004902479650003502528650002502563651001002588651001402598651001302612651001402625084002102639990001502660INLIS00000000001933720250310013106  a0010-0225000023ta250310                |          | |    aARTVET25100 aD H SAWITRI AND A H WARDHANA1 aDetection of Trypanosoma evansi in healthy horses, cattle and buffaloes in East Sumba :beight years after outbreak /cD H SAWITRI AND A H WARDHANA  aSemarang, Indonesia :bIOP Publishing Ltd,c2024  a10 :bill0 aR D W ARDI0 aASWAN0 aK A MARYUNANI ET AL0 aH KOESMARA0 aZ M GAZNUR0 aM AMMAR ET AL.0 aE M SARI0 aM A N ABDULLAH0 aM AMMAR ET AL  aAbstract. Trypanosoma evansi is a widespread hemoflagellate parasite that causes surra (trypanosomiasis) in a variety of mammal species, including horses, cattle, and buffaloes. In 2010-2012, the greatest outbreak of surra in Indonesia occurred on one of Indonesia's most remote islands, Sumba.  This parasite has killed thousands of animals, particularly horses. The purpose of this study was to determine the prevalence of T. evansi infection in healthy livestock (buffaloes, cattle, and horses) after the 8th outbreak in East Sumba using different methods, including serological test (CATT/T. evansi), parasitological test (WBF, Giemsa-stained blood smears (GSBS), and Microhematocrit Centrifugation Test (MHCT), and molecular method by PCR. The survey was conducted during the arid season, when the population of vectors was at its lowest. Blood samples were taken from 111 animals in three subdistricts in East Sumba (Lewa, Wulla Waijelu, and Kota Waingapu). Results revealed that the infection rate of livestock with T. evansi varies by animal species and detection methods. No positive sampel found by Micro Hematocrit Centrifugation Test (MHCT), wet blood film (WBF), and Giemza-stained blood smears (GSBS).  The overall infection prevalence according CATT/T.evansi and PCR were 7.2%( 8/111) and 5.4%  (6/111); respectively. The infection rates by CATT T.evansi and PCR were as follows: horse, 0/57 (0%) and 1/ 57(1.75%); buffalo 5/23 (21.7%) and 13.04% (3/23); cattle, 3/31 (9.7%) and 2/31(6.45%); respectively. Buffalo and cattle have a higher prevalence of T. evansi compared to horses. The results of the present study indicate that cattle and buffalo may serve as a reservoir for T. evansi, which poses a high risk to horses due to the virulence of Surra when vector populations are abundant.  adoi:10.1088/1755-1315/1292/1/012039  aIOP Conf. Series: Earth and Environmental Science 1292 (2024) 012039 4aResearch Centre for Veterinary Science 4aThe National Research and Innovation  Agency 4aFaculty of Veterinary Medicine 4aAirlangga University 4aBogor 4aIndonesia 4aSurabaya 4aIndonesia  aARTVET2510 D H d  aARTVET2510