02600     2200457   4500001002100000005001500021035002000036007000300056008003900059082001500098084002100113100002400134245011100158250003800269260008700307300001300394650000800407650005200415650001400467650001200481700002100493700001900514700001900533700001000552700001800562700001800580700001900598700001700617700002200634700001800656700002100674700002200695700002500717700001800742700002400760700002000784520125300804651001302057856005702070990001502127INLIS00000000001932520250227025250  a0010-0225000011ta250227                |          | |    aARTVET2505  aARTVET2505 AJE d0 aAjeng Fabeane Putri1 aDetection of Very Virulent Infectious Bursal Disease (IBD) in Chicken in  West Java /cAjeng Fabeane Putri  aVol. 18 (1): 37-43, Februari 2024  aBanda Aceh, Indonesia :bFakulitas Kedokteran Hewan Universitas Syiah Kuala,c2024  a7 :bill 4aIBD 4aREVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION 4aISOLATION 4aCHICKEN0 aAgus Winarsongko0 aHeri Hoerudin,0 aGita Sekarmila0 aAhpan0 aJejen Jaelani0 aWawan Gunawan0 aRina Dewiyanti0 aYuda Pratama0 aHarimurti Nuradji0 aNuha Fairusya0 aFitrine Ekawasti0 aRahmat Setya Adji0 aNLP Indi Dharmayanti0 aRisa Indriani0 aBambang Ngaji Utomo0 aSri Suryatmiati  aInfectious Bursal Disease (IBD), also known as Gumboro disease, is an acute, highly contagious disease that infects chickens and causes a high mortality rate of up to 100% in young animals. The disease is caused by Infectious Bursal Disease Virus (IBDV) of the genus Avibirnavirus, family Birnaviridae. The disease has been reported in Indonesia since 1976, and management strategies for the disease, such as vaccination, have been applied to prevent and control outbreaks in poultry farms. In this study, we conducted the detection of the disease in chickens from a farm in West Java with a mortality rate of 80%. Chickens showing clinical signs, such as sudden death, anorexia, watery diarrhea, and ruffled feathers, were necropsied, and organ samples, including the bursa Fabricius, brain, and spleen, were collected. The samples were then tested using Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) to confirm the diagnosis of IBD. Positive results were obtained in this study, highlighting the need for improved biosecurity in poultry farms in Indonesia. These results also provided a basis for further research on viral characterization to develop detection kits or vaccines for IBD using local isolates from the field in Indonesia. 4aINDONESA  aDOI: https://doi.org/10.21157/j.med.vet..v14i2.35819  aARTVET2505