02140     2200181   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100001900128245014400147260004500291300000700336650004200343520157300385INLIS00000000001921320240911014951  a0010-0924000292ta240911                |          | |    aARTVET2418  aARTVET24180 aWardhana AH1,21 aPengembangan Biosensor Penyakit Surra (Trypanosoma evansi)  Berbasis Protein dengan Metode Differential Pulse Voltammetry /cWardhana AH1,2  a1Balai Besar Penelitian Veteriner,c2020  a37 4aPengembangan Biosensor Penyakit Surra  aTrypanosoma evansi remains a major cause of Trypanosomiasis in Indonesia, called 
Surra. However, the lack of accessibility and affordability for diagnostic tests: Card 
Agglutination Test for Trypanosomiasis/T. evansi – CATT/T. evansi; ELISA and PCR lead to 
unsuccessful control of Surra in the field. Accordingly, the accurate, rapid and 
applicable kit for alternative Surra diagnosis is needed, for example biosensor. The study 
aimed to investigate the performance of protein-based biosensor of Surra (Trypanosoma 
evansi) for distinguishing positive and negative sera. Herein, protein was obtained from 
the T. evansi propagation. This protein was immobilized by carbodiimide reaction on the 
surface of carbon screen printed electrode as the lowest interaction material with 
serum/antibody (% I < 90%). The sensor method used in this present study was 
differential pulse voltammetry (DPV). Data were normalized and analyzed using 
PSTrace 5.8 program. The results demonstrated that false negative and false positive of 
sensor were observed at 64 and 2 dilution times respectively. The positive serum 
analyzed using DVP (potential range -0.2 - +1.0 V, scan rate 0.1 Vs-1) revealed that the 
slope of positive serum (1.84) was higher than those of the negative serum (1.01) 
indicating the sensitivity of the produced biosensor for Surra (T. evansi).  Similarly, these 
results were confirmed by serological test (CATT/T. evansi). From this study, the 
obtained protein-based biosensor is a promising tool for Surra detection in livestock.