01982     2200181   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100001600128245014000144260006400284300000700348650002200355520142300377INLIS00000000001921020240911014345  a0010-0924000289ta240911                |          | |    aARTVET2411  aARTVET24110 aEndrawati D1 aMolecular Profile of Trichophyton mentagrophytes and Microsporum canis  based on PCR-RFLP of Internal Transcribed Spacer /cEndrawati D  aIndonesian Research Institute for Veterinary Science,c2020  a12 4aMolecular Profile  aTrichophyton mentagrophytes and Microsporum canis are dermatophytes fungi which commonly infect animal and human. 
Conventional and molecular methods were used for identification of the fungus. The region of internal transcribed spacer (ITS) 
has a high probability for fungal identification. PCR-RFLP was reported as a useful method to differentiate dermatophytes fungi. 
The objective of the study was to compare molecular profile of T. mentagrophytes and M. canis based on the result of ITS 
fragment digestion using Dde I, Hinf I and Mva I. The molds were isolated from skin scrapping of 18 animals which showed 
dermatophytosis lesion. The isolated molds were grown on agar plate for 14 days of incubation at 37oC and then identified based 
on macro and microscopic morphologies. Amplification of chitin synthase gene was used for confirmation and separation of 
dermatophytes from other fungi. ITS fragment was amplified and then digested using restriction enzymes Dde I, Hinf I and Mva 
I. The result showed that digestion products from ITS fragment of T. mentagrophytes and M. canis were different. The fragment 
159 bp from Dde I, 374 bp from Hinf I and 89 bp from Mva I were present in T. mentagrophytes but absent in M. canis.   Based 
on these results, specific RFLP profile of digestion ITS region by Dde I, Hinf I and Mva I can be used as a specific marker for 
species of dermatophytes fungi.