02460     2200181   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100003200128245019100160260006300351300000600414650003100420520182700451INLIS00000000001920920240911014052  a0010-0924000288ta240911                |          | |    aARTVET2407  aARTVET24070 aSri Suryatmiati Prihandani*1 aREKONSTITUSI ISOLAT KERING BEKU BAKTERI Pasteurella multocida PENYEBAB  PENYAKIT NGOROK PADA SAPI DAN IDENTIFIKASI ULANG SECARA  KONVENSIONAL DAN MOLEKULER /cSri Suryatmiati Prihandani*  aKelti Bakteriologi Balai Besar Penelitian Veteriner,c2021  a8 4aREKONSTITUSI ISOLAT KERING  aBeef as a source of animal protein is very popular because it can be processed into various 
menus and has a delicious taste. The availability of beef as a food ingredient of animal origin is 
influenced by health conditions in its maintenance. During the transition season, several endemic diseases 
such as Snoring disease (Septicemia epizootica) caused by Pasteurella multocida can cause production 
disruptions. Therefore it is necessary to know how the morphology and properties of this bacteria. In this 
study, the reconstitution of freeze-dried P. multocida isolates from cows continued with conventional and 
molecular re-identification were carried out to determine their characteristics. After reconstituting on 
Brain Heart Infusion media, subculturing on blood agar media, identification was carried out with 
biochemically step and molecularly step with the Polymerase Chain Reaction technique. The results 
showed that P. multocida isolates had morphologically short stems (coccoid) and Gram negative staining, 
did not grow in Mac Concey agar medium, non motile, oxidase positive, and catalase positive. The 
carbohydrate fermentation test on Triple Sugar Iron Agar media showed that P. multocida isolates could 
ferment glucose, lactose and sucrose but did not produce H2S and CO2 gas. Amplification of specific P. 
multocida genes was carried out using OIE standard primers which gave a PCR product of 460 bp in 
accordance with OIE standards. The conclusions of this study provide information that the conventional 
and molecular reconstitution of P. multocida freeze-dried isolates shows that bacteria are identical to the 
original. Furthermore, it is expected that high economic losses due to snoring in cattle can be anticipated 
by detecting the disease agent as early as possible.