02213     2200193   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100003200128245014400160260001000304300000700314650002600321700002000347520165200367INLIS00000000001920820240911013743  a0010-0924000287ta240911                |          | |    aARTVET2306  aARTVET23060 aDyah Haryuningtyas Sawitri11 aComparative pathology of mice infected with high and low virulence of Indonesian Trypanosoma evansi isolates /cDyah Haryuningtyas Sawitri1  c20120  a12 4aComparative pathology0 aRini Damayanti1  aMice infected with T. evansi cause various
clinical manifestations and histopathological changes. The
aim of this study was to compare the histopathological
lesions of mice infected with T. evansi Bang 87 isolates
(high virulence) and Pml 287 isolates (low virulence). A
total of 15 susceptible mice (DDY) were divided into three
groups (?ve mice/group): Groups I and II each were
infected with 104 T. evansi of high virulence (Bang87) and
low virulence (Pml 287), respectively, whereas group III
served as a control group. A total of three mice from group
I, and one mouse from each group II and III were killed at 4
dpi. A total of two mice from each group II and III were
killed at 24 dpi. Two remaining mice from each group were
observed until succumb. Mice of group I and group II at 4
dpi showed no gross lesions. However, mice of group I
showed very acute animal death at 5 dpi and showed mild
to moderate histopathological lesions at 4 dpi, namely non-
suppurative encephalitis, non-suppurative pneumonia,
hepatitis non-suppurative with intravascular trypanosomi-
asis, tubular degeneration and necrosis. Group II showed
chronic death at 26 dpi with signi?cant gross pathological
changes at 24 dpi in spleen (swelling 10 times than normal
size) accompanied by severe non-suppurative encephalitis,
cholangiohepatitis non-suppurative and bile duct prolifer-
ation, diffused splenic necrosis. The result of this study is
expected to be used as a basis for improved treatment
management in cattle infected with high virulence T.
evansi isolates that are need to be handled appropriately to
avoid fatal consequences.