na INLIS000000000019198 20240911011116 0010-0924000277 ta 240911 | | | ARTVET2392 ARTVET2392 Restriction fragment length polymorphism analysis of genes of virulent strain isolate of Toxoplasma gondii using enzyme DdeI / Fitrine Ekawasti1,2 Vol.7/No.2/7 1. Indonesian Research Center for Veterinary Sciences : International Journal of One Health, 2021 8 Restriction fragment length Umi Cahyaningsih2 N. L. P. Indi Dharmayanti1 Siti Sa’diah3 Didik Tulus Subekti1 Zul Azmi1 Muhammad Ibrahim Desem1 oxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii. The pathogenicity of this species depends on the type originating from the clonal population. Techniques for more accurately determining the type of T. gondii have recently been developed using genetic markers. Specifically, T. gondii has been typed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). This study aimed to identify sets of PCR-RFLP markers that have high power to discriminate genotyping of T. gondii and are easy to use and are easy to use. The objective of this study was to characterize virulent strain isolates of T. gondii by PCR-RFLP using 10 markers with DdeI.