01674     2200253   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113245015300128250001700281260010400298300000600402650003200408700002200440700003100462700002000493700002500513700001400538700002800552520084000580INLIS00000000001919820240911011116  a0010-0924000277ta240911                |          | |    aARTVET2392  aARTVET2392  aRestriction fragment length polymorphism analysis of genes of virulent  strain isolate of Toxoplasma gondii using enzyme DdeI /cFitrine Ekawasti1,2  aVol.7/No.2/7  a1. Indonesian Research Center for Veterinary Sciences :bInternational Journal of One Health,c2021  a8 4aRestriction fragment length0 aUmi Cahyaningsih20 aN. L. P. Indi Dharmayanti10 aSiti Sa’diah30 aDidik Tulus Subekti10 aZul Azmi10 aMuhammad Ibrahim Desem1  aoxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important 
zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii. The 
pathogenicity of this species depends on the type originating from the clonal population. Techniques for more accurately 
determining the type of T. gondii have recently been developed using genetic markers. Specifically, T. gondii has been typed 
using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). This study aimed to identify sets 
of PCR-RFLP markers that have high power to discriminate genotyping of T. gondii and are easy to use and are easy to use. 
The objective of this study was to characterize virulent strain isolates of T. gondii by PCR-RFLP using 10 markers with 
DdeI.