01571     2200217   4500001002100000005001500021035002000036007000300056008003900059082001600098084001600114100001700130245008900147260000900236300000900245650002300254700001900277700001200296700001400308520103100322INLIS00000000001917820240911113339  a0010-0924000257ta240911                |          | |    aARTVERT2186  aARTVERT21860 aSumarningsih1 aRecombinant LipL32 Protein for Leptospirosis Detection in  Indonesia /cSumarningsih  c2015  a8art 4aRecombinant LipL320 aSimson Tarigan0 aSusanti0 aKusmiyati  aLeptospirosis is an endemic zoonotic disease in tropical countries. However, detection using the microagglutination test (MAT) 
as a gold standard is difficult to perform in Indonesia. Therefore, recombinant LipL32 protein (rLipL32) has been studied as an 
antigen for an ELISA test to detect Leptospirosis. We produced rLipL32 using the pRSET-C vector and expressed it in E. coli 
BL21 (DE3) competent cells. Under native conditions, we purified 2 mg rLipL32 protein from 500 ml culture. Analysis using 
western blotting and ELISA showed that serum from the bovine positive MAT serovar Hardjo could recognize pure rLipL32 
protein. This result confirms an earlier study that indicates that rLipL32 protein is a good antigen for Leptospirosis detection. The 
diagnostic assay using rLipL32 is safe because it does not use infectious bacteria as an antigen and because it is easy to perform 
in every diagnostic laboratory in Indonesia. However, further study is still required for field validation of the rLipL32 assay.