02240     2200193   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100001800128245009600146260012900242300000600371650003100377700002500408520161300433INLIS00000000001914620240911082921  a0010-0924000225ta240911                |          | |    aARTVET1814  aARTVET18140 aRISA INDRIANI1 aANTIBODY LEVEL OF AVIAN INFLUENZA SUBTYPE H5   IN COMMERCIAL BROILER FARMS /cRISA INDRIANI  aInstitute Centre Research of Veterinary :bInternational Conference on Livestock Production and Veterinary Technology,c2012  a5 4aAVIAN INFLUENZA SUBTYPE H50 aN.L.P.I. DHARMAYANTI  aHighly Pathogenic Avian Influenza (HPAI) subtype H5N1 has been in Indonesia since the middle of 
2003. Vaccination of chickens has become a routine practice in the countries of Asia (including Indonesia) 
where HPAI is endemic. This is especially conducted for layer and breeder farms, but the broilers are often 
left unvaccinated. To observe the innate immune antibodies against AI subtype H5, which derived from hens 
in young broilers, this experiment was conducted on commercial broiler farms in sector 3. Seventeen farms 
which located in the district of Sukabumi and Cianjur were chosen. Twenty serum samples were collected 
randomly from each farm by random at the time of broiler day old chicks (DOC), two weeks of age and at 
around four to five weeks of age or market ready. Serum samples were tested by using haemagglutination 
inhibition (HI) against three antigen isolates of AI subtype H5N1 and one antigen of subtype AI H5N2. Data 
from HI tests were analyzed as the geometric mean titre (GMT) and negative titre (< 16) was regarded as 4 
for the calculation of GMT. Results of analysis of serum samples showed that DOC of broiler chickens had 
maternal antibody titre of AI subtype H5 were ? 16, then at 2 weeks of age the maternal antibody titre 
subtype H5 were < 16 and at the time market ready or at 4 – 5 weeks of age the maternal antibody titre AI 
subtype H5 has reached 0. It is concluded that maternal antibody against AI subtype H5 in broiler is no longer 
providing protection, and it is possible that broiler chickens could be infected with AI virus in the field.