02492     2200229   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100002500128245015000153250001800303260004600321300000600367650002300373700002100396700001800417520181200435990001502247INLIS00000000001908720250203091844  a0010-0924000166ta250203                |          | |    aARTVET1657  aARTVET16570 aDYAH HARYUNINGTYAS 11 aVariabilitas Sekuen Gen Tubulin ? Isotipe-1 Cacing Haemonchus contortus Isolat Resisten terhadap Benzimidazole pada Domba /cDYAH HARYUNINGTYAS 1  aVol. 11 No. 3  aBalai Penelitian Veteriner :bJITV,c2006  a6 4aSekuen Gen Tubulin0 aWAYAN T. ARTAMA20 aWIDYA ASMARA2  aHARYUNINGTYAS, D., W.T. ARTAMA and W. ASMARA. 2006. Sequence variability of the ? Tubulin isotype-1 gene in 
benzimidazole resistant strains of Haemonchus contortus, a nematode parasite of sheep. JITV 11(3): 235-240. 
Resistance to benzimidazole at a population of gastrointestinal nematode, especially Haemonchus contortus is a widespread 
problem. Therefore, anthelmintic will not effective anymore. Benzimidazole resistance in nematode has been shown to be 
heritable and defined as an increase in frequency of individual worms able to drug tolerate. In Indonesia resistance to 
anthelmintic albendazole has been reported on sheep in district of Bogor, Kuningan, Kendal and province of Yogyakarta. 
Molecular mechanism of resistance to benzimidazole on nematode and fungi related to single gene which code ß tubulin. The 
aim of this research is to determine a genetics variability of isotype-1 ß tubulin genes from Indonesian strain of H. contortus 
which resistant to benzimidazole. Sequencing were carried out at a 520 bp fragment of the isotipe-1 ß tubulin gene of six H. 
contortus resistant strain from four sheep from SPTD Trijaya farm, district of Kuningan, West Java; UPTD Pelayanan Kesehatan 
Hewan farm, district of Bantul, Yogyakarta respectively. One H. contortus susceptible strains as a control come from farmer 
from Cicurug, district of Sukabumi, West Java. Results indicated that there are genetics variability of fragment isotype-1 ß 
tubulin genes from H. contortus from UPTD Pelayanan Kesehatan Hewan farm, Bantul, Yogyakarta (H1, H2, and C2). There are 
no genetics variability in the fragment gene of H. contortus resistant strain from SPTD Trijaya farm, Kuningan, West Java (Kn1, 
Kn2 and K1). Genetic variability occur between 2 isolate from different location.  aARTVET1657