02032     2200217   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100002300128245011900151250002100270260005200291300000600343650002300349700002400372520140300396990001501799INLIS00000000001906920250203084621  a0010-0924000148ta250203                |          | |    aARTVET1430  aARTVET14300 aDYAH HARYUNINGTYAS1 aDeteksi Mikrofilaria/Larva Cacing Brugia malayi pada Nyamuk dengan Polimerase Chain Reaction /cDYAH HARYUNINGTYAS  aVol. 13 No .3 A.  aBalai Besar Penelitian Veteriner :bJJTV,c2008  a9 4aMikrofilaria/Larva0 aDIDIK TULUS SUBEKTI  aLymphathic filariasis that is also known as elepanthiasis is caused by infestation of 3 species nematode Wuchereria
bancrofti, Brugia malayi and Brugia timori. In Indonesia 70% filariasis case caused by Brugia malayi. Mosquito species from
genus Anopheles, Aedes, Culex, Mansonia and Armigeres are known as vector of this disease. Microfilaria detection
mosquito is one methode to know infection rate in vector population in endemic area.The objectives of the research were
study the ability of Hhal repeat applicable to detect microfilaria/larvae in a pool of mosquitoes and to get description of adult
mosquito night biting population lived in endemic area of filariasis brugian. Mosquito as positive control used in this research
come from laboratory of parasitology of FKUI. Mosquito sample from the field was from Binawara and Kolam Kiri villages
South Kalimantan province. Mosquito were trapped then identified by its species. DNA of mosquitoes was extracted and then
run by the PCR using Hha 1 repeat primer. Result of the research indicated that adult mosquitoes night biting from Binawara
village consist of Culex, Mansonia, Anopheles genus and from Kolam Kiri village only from Mansonia genus. Hha 1 repeat
primer is applicable to detect 1 mosquito infected with microfilaria/larvae in a pool of negative mosquitoes. Mosquito samples
from the two villages showing negative PCR.  aARTVET1430