01991     2200205   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100001700128245011900145260009900264300000600363650002000369700001700389520136400406990001501770INLIS00000000001905820250203083005  a0010-0924000137ta250203                |          | |    aARTVET2341  aARTVET23410 aSITI CHOTIAH1 aVIABILITAS CLOSTRIDIUM SPP. SETELAH KONSERVASI  EKS SITU DALAM JANGKA WAKTU LAMA   PADA SUHU KAMAR /cSITI CHOTIAH  aBalai Besar Penelitian Veteriner :bSeminar Nasional Teknologi Peternakan dan Veteriner,c2007  a8 4aCLOSTRIDIUM SPP0 aLILY NATALIA  aA wide variety of techniques are used for the preservation of microbes and it may be difficult to choose 
the most suitable for a particular microbes. The preservation method used should minimize loss of viability 
during processing and storage so that after preservation, cultures will survive for long periods. Clostridium 
spp. viability after ex situ conservation by freeze-drying technique with Robertson's cooked meat medium as 
preservation medium in long storage period at room temperature have been evaluated for achieving the 
suitable and efficient monitoring in the microbial germ plasma preservation. A total of 376 dried culture 
samples of 115 Clostridium spp. collection consist of 27 species prepared in vacuum glass ampoules, stored 
for more than 10 years at room temperature have been evaluated for their viability. Each sample was grown 
on specific medium and identified for the bacterial species and type. The results showed that 9 of 115 
collections, representing 7 different species failed to grow during 12 to 19 years storage. That 7 species were 
able to grow during 21 to 30 years ex situ conservation by freeze-drying technique with mist. desiccans 
preservation medium. It is suggested that mist. desiccans preservation medium was better be used in freeze-
drying technique to conserve Clostridium spp. germ plasma.  aARTVET2341