02180     2200217   4500001002100000005001500021035002000036007000300056008003900059082001500098084001500113100002100128245014100149260009000290300000700380650003400387700001000421700001400431520150200445990001501947INLIS00000000001898520250109103520  a0010-0924000064ta250109                |          | |    aARTVET0761  aARTVET07610 aGOZALI R. MOEKTI1 aEVALUASI MENGENAI SPESIFISTTAS ANTIBODI MONOKLONAL K99 TERHADAP ANTIGEN PERLEKATAN ESCHERICHIA COLI ENTEROTOKSIGENIK /cGOZALI R. MOEKTI  aBalai Penelitian Veteriner, Bogor :bProsiding Seminar Teknologi Veteriner 7h.,c1994  a12 4aENGENAI SPESIFISTTAS ANTIBODI0 aSUPAR0 aKUSMIYATI  aIn attempt to develop an improved diagnostic tool for neonatal colibacillosis in calves, monoclonal antibodies (McAb)
directed against K99-adhesin derived from a reference strain of enterotoxigenic Escheridda con (Compton, K12; K99) were
produced. Specificity of McAb produced was evaluated using a slide conglutination teat, indirect enzyme-linked
immunosothent assay (ELISA), and immunoblotting. Antigens used for the specificity testing were prepared from known E.
coif K99 of bath homologous (Compton, KU; K99) and heterologous (841), field-isolates (BL413, G1231, G1150 and
2631), and those of known as 1(99-negative E. coil (i.e. types 1(88, F41 and 987P). The present study resulted in a panel of
20 hybridomas secreting antibodies specific to K99-adhesin. Two of the total hybridomas obtained, 11(99-B5 and 1K99-C9,
were cloned consecutively producing 14 and 10 clones, which were then found to be stabile up to the second cloning. Of 24
McAbs produced from the total clones, three antibodies were tested using a slide agglutination test and proved to be reactive
to K99 antigens prepared either from reference strains or field-isolates, but showed not to be reactive to 1(99-negative E. ca.
Based on the indirect-ELISA, all McAbs produced so far were indicated to be only recognise 1(99 antigens. Moreover
immunolgotting utukttaken in this study demonstrated that the 24 McAbs identify 1(99 antigen located at a molecular weight
marker ranging from 16000 to 17,000 &ikons (D).  aARTVET0761