01881     2200205   4500001002100000005001500021035002000036007000300056008003900059082001400098084001400112100001400126245015400140260009000294300000700384650002400391700001000415700001600425520123400441INLIS00000000001898420240906113625  a0010-0924000063ta240906                |          | |    aARTVET759  aARTVET7590 aKUSMIYATI1 aKLONING MOLEKUL DNA GENOMIK ESCHERICHIA COL' ENTEROTOKSIGENIK 98W :bSEBUAH KAJIAN MENGENAI PENGEMBANGAN VAKSIN SECARA REKAYASA GENETIKA /cKUSMIYATI  aBalai Penelitian Veteriner, Bogor :bProsiding Seminar Teknologi Veteriner 7h.,c1994  a11 4aMOLEKUL DNA GENOMIK0 aSUPAR0 aG.R. MOEKTI  aIn order to analyse DNA fragments encoding synthesis of adhesin antigens in enterotoxigenic Escherichia cell 987P, a
blind molecular cloning was undertaken. Genomic DNA was extracted from field isolates of E. coil bearing 987P adhesin,
and fragmented utilizing restriction enzymes Sau3A or EcoRV. The fragmented genomic DNA was then used to generate
libraries in the E. coil XL1-Blue expression system employing phagemid pBluescript II SK vector. AU clones suspected
recombinant DNA were grown on selective medium for propagating 987P adhesin antigen. Six recombinat clones (mutants)
were selected to be tested serologically using antiserum anti 987P adhesin antigen raised in rabbits. Of the 15 mutants, 13 of
which were found to be able to express a homologous polypeptide compound against 987P anti adhesin antibody. The present
study may therefore suggest that a DNA fragment clone is located in the area of coding sequence for the synthesis of adhesin
antigen in enterotoxigenic Escherichia cob 987P. Further studies may be required particularly in connection to the
determination of the sequence of DNA fragment clone, expression of gene clone, and the potential use of expressing
components as sub-unit vaccines.