03551     2200253   4500001002100000005001500021035002000036007000300056008003900059082001400098084001400112100001000126245016500136250001700301260007400318300000600392650001800398700001700416700001300433700001900446700002500465700001100490520279600501INLIS00000000001896920240906100516  a0010-0924000048ta240906                |          | |    aARTVET592  aARTVET5920 aSUPAR1 aPENGEMBANGAN VAKSIN KOLERA UNGGAS :bI. PROTEKSI VAKSIN  PASTEURELLA MULTOCIDA ISOLAT LOKAL PADA AYAM  TERHADAP UJI TANTANG GALUR HOMOLOG DAN HETEROLOG /cSUPAR  aVol. 6 No. 1  aBalai Penelitian Veteriner :bJurnal Ilmu Ternak dan Veteriner,c2001  a9 4aVAKSIN KOLERA0 aYUDI SETIADI0 aDJAENURI0 aNINA KURNIASIH0 aBHAKTI POERWADIKARTA0 aSJAFEI  aPasteurella multocida locally isolated from chicken and ducks (BCC 299, BCC 2331, DY1, DY2, 12TG, 15TG) and 
imported strains (BCC 1359, 1362; HEDDLESTON group 1 and 6 respectively) had been tested for its pathogenicity in the 
previous study. The aims of this experiment were to study the preparation of local isolate pasteurellosis vaccines and to 
determine the protective effect of that vaccines in chicken against the highly pathogenic local isolates of P. multocida. Killed 
monovalent, bivalent and polyvalent pasteurellosis vaccines were prepared and each was adjunvanted with aluminum hydroxide 
gel at a final concentration of 1.5% and the cell concentration was equal to the No 10 of MacFarland tube standard. Each of the 
vaccine prepared was used to vaccinated on a group of six week old of layer chicken (8 per group). Each chicken was 
subcutaneously injected with 0.2 ml of vaccine, four weeks later each was boostered with similar vaccine with the same dose. 
Two weeks after giving the boostered vaccine each group of chicken were challenged, half with life bacterium of P. multocida 
BCC 2331 and other with DY2. Any chick which survive after challenge was designated as protected by vaccination. Before 
vaccination 1 ml of blood was drawn from each of chicken and then two weeks apart up to challenge. Serum from each sample 
was separated and kept in deep freeze until tested by enzyme-linked immunosorbent assay (ELISA). Chicken vaccinated with 
killed whole cell P. multocida vaccines of monovalent (BCC 2331 or DY2) and bivalent (BCC 2331 + DY2) were protected 
against challenge with live bacterium of either BCC 2331 or DY2 at rate 67-100%. There was no protection in chicken 
vaccinated with either BCC 299, DY1, 12TG, 15TG, BCC 1359, or 1362 killed vaccine. Similarly no protection of chicken 
vaccinated with either DY1 + BCC299, 12TG + 15TG or BCC 1359 + BCC 1362 bivalent vaccines. The protection rate of the 
polyvalent local isolate vaccine was at average 50-75%. All vaccinated chicken showed the presence of antibody responses 
againsted the extract cell and whole cell antigens of either P. multocida BCC 2331 or DY2 local isolate as detected by ELISA. 
The antibody responses from vaccinated chicken against extra cellular antigens prepared from broth cultures of BCC 2331 and 
DY2 were detected only from vaccinated chicken with vaccine containing killed antigen of BCC 2331 and/or DY2 isolate. It is 
likely, the local isolate of P. multocida BCC 2331 and DY2 would be benefit for producing inactive fowl cholera vaccine use in 
Indonesia, but the protective antigen that enhances immune protection should be determined by means of immunoblotting 
techniques. 
Key words: Pasteurella multocida, fowl cholera, vaccine, protection