NINING HARTANINGSIH text Bali Cattle Disease Investigation Unit - IFAD, Denpasar, Bali, Indonesia Penyakit Hewan 1993 Vol. XXV No.46A, Edisi Khusus

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7 Hartaningsih, N., G. E. Wilcox, M. Tenaya and S. So eharsono. 1993. Development of an ELISA for the detectioti of antibodies to Jembrana disease in Bali cattle. Penyakit Hewan 25 (46A): 37-43. An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of antibodies specific for the Jembrana disease agent. Virus present in plasma of experimentally infected cattle and purified by sucrose density gradient centrifugation was used as antigen. In experimentally infected Bali cattle, antibody was detected as early as 8 weeks after infection and the majority of cattle were antibody positive 3 months after infection. The antibody persisted for more than 59 weeks. A serological survey was conducted of cattle from various areas in Indonesia to determine the distribution and prevalence of cattle with antibodies to Jembrana disease virus. Antibodies were detected in serum samples from cattle from five provinces, Bali, Lam pung, East Java (Banyuwangi), West Sumatera and South Kalimantan. This finding correlated with the reported occurrence of clinical Jembrana disease. Key words: ELISA, antibo di, Jembrana disease, Bali cattle NINING HARTANINGSIH DEVELOPMENT ARTVET369 ARTVET369 240905 20240905104123 INLIS000000000018938 Converted from MARCXML to MODS version 3.5 using MARC21slim2MODS3-5.xsl (Revision 1.106 2014/12/19)