01864     2200229   4500001002100000005001500021007000300036008003900039035002000078082001400098084001400112100002400126245011800150250003400268260010200302300000600404520115900410650001601569700001701585700001401602700001801616INLIS00000000001893820240905104123ta240905                |          | |    a0010-0924000017  aARTVET369  aARTVET3690 aNINING HARTANINGSIH1 aDEVELOPMENT OF AN ELISA FOR THE DETECTION OF ANTIBODIES TO JEMBRANA DISEASE IN BALI CATTLE /cNINING HARTANINGSIH  aVol. XXV No.46A, Edisi Khusus  aBali Cattle Disease Investigation Unit - IFAD, Denpasar, Bali, Indonesia :bPenyakit Hewan,c1993  a7  aHartaningsih, N., G. E. Wilcox, M. Tenaya and S. So eharsono. 1993. Development of an ELISA for the detectioti of antibodies to Jembrana disease in Bali cattle. Penyakit Hewan 25 (46A): 37-43. An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of antibodies specific for the Jembrana disease agent. Virus present in plasma of experimentally infected cattle and purified by sucrose density gradient centrifugation was used as antigen. In experimentally infected Bali cattle, antibody was detected as early as 8 weeks after infection and the majority of cattle were antibody positive 3 months after infection. The antibody persisted for more than 59 weeks. A serological survey was conducted of cattle from various areas in Indonesia to determine the distribution and prevalence of cattle with antibodies to Jembrana disease virus. Antibodies were detected in serum samples from cattle from five provinces, Bali, Lam pung, East Java (Banyuwangi), West Sumatera and South Kalimantan. This finding correlated with the reported occurrence of clinical Jembrana disease.
Key words: ELISA, antibo di, Jembrana disease, Bali cattle 4aDEVELOPMENT0 aG. E. WILCOX0 aM. TENAYA0 aS. SOEHARSONO