02191     2200457   4500001002100000005001500021035002000036007000300056008003900059020001500098084001500113100002500128245014200153250003900295260000900334300001300343650001700356650002900373650002300402650002700425650001700452650001700469700002000486700002100506700001800527700001800545700002600563700002200589700002100611700002900632700002200661700002500683700002100708700002400729700002200753700002100775700002100796700002000817520086500837856003101702INLIS00000000001875820240212082133  a0010-0224000008ta240212                |          | |    aARTVET2384  aARTVET23840 aBlacksell, Stuart D.1 aThe Biosafety Research Road Map :bThe Search for Evidence to Support Practices in the Laboratory—Shigella spp. /cBlacksell, Stuart D.  aApplied Biosafety, Vol, 28, No, 2.  c2023  a96–101 4aShigella spp 4apathogen characteristics 4abiosafety evidence 4aknowledge gap analysis 4adisinfection 4ainactivation0 aDhawan, Sandhya0 aKusumoto, Marina0 aLe, Kim Khanh0 aDavis, Ben J.0 aSummermatter, Kathrin0 aO’Keefe, Joseph0 aKozlovac, Joseph0 aAlmuhairi, Salama Suhail0 aSendow, Indrawati0 aScheel, Christina M.0 aAhumibe, Anthony0 aMasuku, Zibusiso M.0 aBennett, Allan M.0 aKojima, Kazunobu0 aHarper, David R.0 aHamilton, Keith  aIntroduction: Shigella bacteria cause shigellosis, a gastrointestinal infection most often acquired from con
taminated food or water.
Methods: In this review, the general characteristics of Shigella bacteria are described, cases of laboratory
acquired infections (LAIs) are discussed, and evidence gaps in current biosafety practices are identi?ed.
Results: LAIs are undoubtedly under-reported. Owing to the low infectious dose, rigorous biosafety level
2 practices are required to prevent LAIs resulting from sample manipulation or contact with infected sur
faces.
Conclusions: It is recommended that, before laboratory work with Shigella, an evidence-based risk assess
ment be conducted. Particular emphasis should be placed on personal protective equipment, handwash
ing, and containment practices for procedures that generate aerosols or droplets.  aDOI: 10.1089/apb.2022.0046