02687     2200445   4500001002100000005001500021035002000036007000300056008003900059020001500098084001500113100002500128245017100153250003900324260000900363300001200372650002300384650002400407650002900431650002300460650002800483700002000511700002100531700001800552700002600570700002200596700002100618700002900639700002200668700001600690700001100706700002100717700002400738700002200762700002100784700002100805700002000826520136400846856003102210INLIS00000000001875620240207031934  a0010-0224000006ta240207                |          | |    aARTVET2382  aARTVET23820 aBlacksell, Stuart D.1 aThe Biosafety Research Road Map :bThe Search for Evidence to Support Practices in the Laboratory—Bacillus anthracis and Brucella melitensis /cBlacksell, Stuart D.  aApplied Biosafety, Vol, 28, No, 2.  c2023  a72–86 4aBacillus anthracis 4aBrucella melitensis 4apathogen characteristics 4abiosafety evidence 4abiosafety knowledge gap0 aDhawan, Sandhya0 aKusumoto, Marina0 aLe, Kim Khanh0 aSummermatter, Kathrin0 aO’Keefe, Joseph0 aKozlovac, Joseph0 aAlmuhairi, Salama Suhail0 aSendow, Indrawati0 aM Christina0 aScheel0 aAhumibe, Anthony0 aMasuku, Zibusiso M.0 aBennett, Allan M.0 aKojima, Kazunobu0 aHarper, David R.0 aHamilton, Keith  aIntroduction: Brucella melitensis and Bacillus anthracis are zoonoses transmitted from animals and animal
products. Scienti?c information is provided in this article to support biosafety precautions necessary to
protect laboratory workers and individuals who are potentially exposed to these pathogens in the workplace
or other settings, and gaps in information are also reported. There is a lack of information on the appropriate
effective concentration for many chemical disinfectants for this agent. Controversies related to B. anthracis
include infectious dose for skin and gastrointestinal infections, proper use of personal protective equipment
(PPE) during the slaughter of infected animals, and handling of contaminated materials. B. melitensis is
reported to have the highest number of laboratory-acquired infections (LAIs) to date in laboratory workers.
Methods: A literature search was conducted to identify potential gaps in biosafety and focused on ?ve main
sections including the route of inoculation/modes of transmission, infectious dose, LAIs, containment
releases, and disinfection and decontamination strategies.
Results: Scienti?c literature currently lacks information on the effective concentration of many chemical dis-
infectants for this agent and in the variety of matrices where it may be found. Controversies related to  aDOI: 10.1089/apb.2022.0042