03669     2200421   4500001002100000005001500021035002000036007000300056008003900059084001500098100002500113245018300138250003900321260000900360300001400369650003100383650002900414650002300443650002800466650002800494700002000522700002100542700001800563700002600581700002200607700002100629700002900650700002200679700002500701700002100726700002400747700002200771700002100793700002100814700002000835520236100855856003103216INLIS00000000001875320240207031818  a0010-0224000003ta240207                |          | |    aARTVET23790 aBlacksell, Stuart D.1 aThe Biosafety Research Road Map :bThe Search for Evidence to Support Practices in the Laboratory—Zoonotic Avian In?uenza and Mycobacterium tuberculosis /cBlacksell, Stuart D.  aApplied Biosafety, Vol,  28, No, 3  c2023  a135–151 4aMycobacterium tuberculosis 4apathogen characteristics 4abiosafety evidence 4abiosafety knowledge gap 4aZoonotic avian in?uenza0 aDhawan, Sandhya0 aKusumoto, Marina0 aLe, Kim Khanh0 aSummermatter, Kathrin0 aO’Keefe, Joseph0 aKozlovac, Joseph0 aAlmuhairi, Salama Suhail0 aSendow, Indrawati0 aScheel, Christina M.0 aAhumibe, Anthony0 aMasuku, Zibusiso M.0 aBennett, Allan M.0 aKojima, Kazunobu0 aHarper, David R.0 aHamilton, Keith  aAbstract
Introduction: The Biosafety Research Road Map reviewed the scienti?c literature on a viral respiratory path-
ogen, avian in?uenza virus, and a bacterial respiratory pathogen, Mycobacterium tuberculosis. This project
aims at identifying gaps in the data required to conduct evidence-based biorisk assessments, as described
in Blacksell et al. One signi?cant gap is the need for de?nitive data on M. tuberculosis sample aerosolization
to guide the selection of engineering controls for diagnostic procedures.
Methods: The literature search focused on ?ve areas: routes of inoculation/modes of transmission, infectious
dose, laboratory-acquired infections, containment releases, and disinfection and decontamination methods.
Results: The available data regarding biosafety knowledge gaps and existing evidence have been collated
and presented in Tables 1 and 2. The guidance sources on the appropriate use of biosafety cabinets for spe-
ci?c procedures with M. tuberculosis require clari?cation. Detecting vulnerabilities in the biorisk assessment for
respiratory pathogens is essential to improve and develop laboratory biosafety in local and national systems.Abstract
Introduction: The Biosafety Research Road Map reviewed the scienti?c literature on a viral respiratory path-
ogen, avian in?uenza virus, and a bacterial respiratory pathogen, Mycobacterium tuberculosis. This project
aims at identifying gaps in the data required to conduct evidence-based biorisk assessments, as described
in Blacksell et al. One signi?cant gap is the need for de?nitive data on M. tuberculosis sample aerosolization
to guide the selection of engineering controls for diagnostic procedures.
Methods: The literature search focused on ?ve areas: routes of inoculation/modes of transmission, infectious
dose, laboratory-acquired infections, containment releases, and disinfection and decontamination methods.
Results: The available data regarding biosafety knowledge gaps and existing evidence have been collated
and presented in Tables 1 and 2. The guidance sources on the appropriate use of biosafety cabinets for spe-
ci?c procedures with M. tuberculosis require clari?cation. Detecting vulnerabilities in the biorisk assessment for
respiratory pathogens is essential to improve and develop laboratory biosafety in local and national systems.  aDOI: 10.1089/apb.2022.0038