02512     2200349   4500001002100000005001500021035002000036007000300056008003900059084001500098100002000113245013500133250004800268260000900316300001200325650001200337650001000349650000800359650000700367650000700374650000900381700002000390700001900410700001700429700002000446700001900466700002200485700003400507520156200541856004502103651001402148INLIS00000000001875120240207030911  a0010-0224000001ta240207                |          | |    aARTVET23770 aHartawan, Risza1 aRetrospective analysis of Marek's disease virus  outbreak in 2013 on chicken farms in North Sumatra,  Indonesia /cHartawan, Risza  aLivestock and Animal Research, Vol.21, No.3  c2023  a170-180 4aMarek's 4aMDV-1 4aMeq 4agC 4agE 4app380 aNurjanah, Diana0 aSuyatno, Teguh0 aPurwany, Any0 aRatnawati, Atik0 aIndriani, Risa0 aSendow, Indrawati0 aDharmayanti, Ni Luh Putu Indi  aObjective: Marek's disease is lymphoma in chicken caused by Marek's disease virus serotype 1 
(MDV-1), characterized by paralysis and tumour formation. The disease has caused enormous 
economic loss worldwide, including in Indonesia. Unfortunately, molecular data related to MDV-1 
from Indonesia is limited. Therefore, this study aimed to identify genetic characteristics of MDV-1 
causing outbreaks in commercial farms in North Sumatra, Indonesia, 2013. 
Methods: The amplification of Meq, pp38, gC, and gE genes of the strain MDN2013 was carried out 
using the PCR protocol. Fragment gene sequencing was accomplished using the Sanger method. 
Subsequently, the bioinformatic analyses of BLASTN and phylogenetic tree were applied to analyze 
the molecular characteristics of these gene sequences. 
Results: The Meq, pp38, gC, and gE genes of the strain MDN2013 were successfully sequenced and 
submitted to the NCBI GenBank database. The Meq genes share a high identity with Asian viruses, 
mainly those originating from Indonesia, India, and China. The pp38, gC, and gE genes share 
similarities with other viruses in more diverse regions in Asia, Africa, Europe, and America. The 
phylogenetic tree analysis of the Meq gene demonstrated specific patterns for pathotype biodiversity 
where the strain MDN2013 has been categorized as virulent up to very virulent virus. 
Conclusion: In conclusion, the molecular approach is an effective tool for investigating the MDV-1 
biodiversity in the field, especially the Meq gene sequence analysis  ahttps://doi.org/10.20961/lar.v21i3.78210 4aIndonesia