02477     2200289   4500001002100000005001500021035002000036007000300056008003900059084001500098100001900113245023300132250002100365260000900386300003000395650001300425650001700438650001200455650001300467650001700480700002900497700001300526700001100539520158200550600001702132856003802149INLIS00000000001807120220224122738  a0010-0222000045ta220224                |          | |    aARTVET23851 aSinaga Edelina1 aNanokitosan Efektif Menekan Jumlah Bakteri Gram Negatif dan Gram Positif :b(NANOCITOSAN EFFECTIVELY SUPPRESSES BACTERIA GRAM NEGATIVE AND GRAM POSITIVE CAUSES OF ENDOMETRITIS IN FRIESIAN HOLSTEIN COW IN VITRO) /cSinaga Edelina  aJurnal Veteriner  c2021  aVol. 22 No. 2 : 198 - 206 4abacteria 4aendometritis 4aFH cows 4ain vitro 4ananochitosan1 aKarja Kurniani, Ni Wayan1 aAndriani1 aAmrozi  aThis study was conducted to isolate and identify the caused bacteria of endometritis in Frisian
Holstein (FH) and assessed the effectiveness of nanochitosan against bacterial endometritis in vitro.
Sample was collected from six FH cows with subclinical endometritis was examined by ultrasonography,
then was calculated the number of colonies, isolation and identification of bacteria. Nanochitosan was
calculated the protein content and made into neutral pH with concentrations of 0,5%, 1% and 2%. The
results showed that the highest total plate count in the endometrial fluid was founded at number 626 with
4,7 ± 0,6 Log cells/mL and the lowest was found at number 532 with 3,3 ± 0,8 Log cells/mL. Bacteria in the
Endometritis fluid was founded Gram-negative bacteria were Escherichia coli, Pseudomonas aeruginosa,
and Klebsiella sp. Furthermore, Gram-positive bacteria were founded Staphylococcus aureus, Bacillus sp.
and S. pyogenes. The challenge test of nanochitosan 1% and 2% with E. coli showed insignificant results
when compared with positive controls (P>0,05). Nanochitosan with all concentrations can reduced number
colonies of P. aeruginosa and Bacillus sp. significantly compared with negative controls (P<0,05). The
challenge test of nano chitosan 0,5% and 2% were able to reduced the number of S. aureus and Klebsiella sp.
colonies but could not reduce of S. pyogenes. These findings showed treatment of nanochitosan as an
antibacterial is able to reduce the number of E. coli, Klebsiella sp. and P. aeruginosa and Bacillus sp. and
S. aureus 4aendometritis  a10.19087/jveteriner.2021.22.2.198