03493     2200217   4500001002100000005001500021035002000036008004100056041000700097082001200104090001200116100002100128700002100149700002200170700001400192245019600206260000900402300002300411500279300434650004803227INLIS00000000000196620240216034335  a0010-0521001966240216                |          |        aid  a--/1241  a--/12410 aSarosa, Antonius0 aSyafriati, Tatty0 aADJID, R.M. Abdul0 aYuningsih1 aPenyakit rabies di Indonesia dan pengembangan teknik diagnosisnya. / Adjid, R.M. Abdul ; Sarosa, Antonius ; Syafriati, Tatty ; Yuningsih (Balai Penelitian Veteriner Bogor) /cSarosa, Antonius  c2005  aV .15(4) p.165-172  aWartazoa .-- V .15(4) p.165-172.-- ABSTRAK : Rabies is a zoonotic disease which is crucial for public health, as it can infect human beings and causes death . Rabies has spread across the world including Indonesia . Control to erradicate rabies still faces many obstacles . Rabies research at Research Institute for Veterinary Science emphasizes on rabies erradication focussing on some aspects of diagnostic techniques . Studies on the spesimen preservation, the shipment to the laboratory and the collection of brain specimens by straw method as well as the use of cornea touch preparat as intra vitam specimens have been conducted. Rabies diagnostic techniques by NPLA or modified FAVN on multispot slides and their applications in the field have also been studied . This paper also describes the preparation of FITC conjugate. ELISA technique was developed for serological test but still need to be improved to reduce non specific reaction, while RREID was used for antigen detection . Rabies diagnostic was conducted by using RT-PCR but its application was very difficult to be done for intra vitam . Research on Croton tiglium and Pangium edule extracts as an alternative for the strychnine poison used for dog elimination in the field has also been conducted but further research is needed in the future . / Penyakit rabies merupakan penyakit zoonosis yang sangat penting artinya bagi kesehatan masyrakat, karena dapat mengakibatkan kematian pada penderitanya. Penyakit rabies tersebar di berbagai negara termsuk Indonesia. Upaya pemberantasan rabies yang dilakukan masih banyak mengalami kendala. Hasil-hasil penelitian yang dilakukan di Balai Penelitian Veteriner dapat menunjang pemberantasan rabies di Indonesia, terutama dalam hal teknik diagnosis. Berbagai aspek penelitian rabies yang dilakukan yaitu penggunaan zat pengawet spesimen, cara pengirimannya serta pengambilan otak anjing dengan metode straw; penggunaan preparat sentuh kornea mata sebagai spesimen intra vitam; teknik neutralization peroxidase linked assay (NPLA) atau modifikasi uji fluorescent antibody virus neutralization test (FAVN) pada multispot slide, aplikasinya di lapang; deteksi antigen dengan penggunaan rapid rabies enzyme immunodiagnostic (RREID); serta pembuatan konjugat fluorescent isothiocyanate (FITC). Uji enzyme-linked immunosorbent assay (ELISA) untuk deteksi antibodi rabies telah dikembangkan, namun masih dijumpai adanya reaksi tidak spesifik. Teknik reverse transcriptase polymerase chain reaction (RT-PCR) telah diteliti, namun aplikasinya sulit dilaksanakan pada spesimen intra vitam. Demikian halnya dengan racun ekstrak biji kemalakian dan ekstrak biji picung juga diteliti sebagai pengganti strychnine untuk eliminasi anjing di lapang, namun masih diperlukan penelitian lanjutan. 4aRABIES-DIAGNOSTIC TECHNIQUES-DOGS-INDONESIA