01724     2200205   4500001002100000005001500021035002000036245016000056700002000216700001200236700004900248250002300297260000900320300001700329084001500346082001500361008003900376520108800415990001501503INLIS00000000001782520210722101513  a0010-0721002357  aRecombinant LipL32 Protein for Leptospirosis Detection in Indonesia /cSumarningsih; Tarigan, Simson; Susanti; Kusmiyati (Balai Besar Penelitian Veteriner)  aTarigan, Simson  aSusanti  aKusmiyati (Balai Besar Penelitian Veteriner)  aProcedia Chemistry  c2016  aVol.18. 2016  aARTVET2098  aARTVET2098210722                |          | |    aLeptospirosis is an endemic zoonotic disease in tropical countries. However, detection using the microagglutination test (MAT) 
as a gold standard is difficult to perform in Indonesia. Therefore, recombinant LipL32 protein (rLipL32) has been studied as an 
antigen  for  an  ELISA  test  to  detect  Leptospirosis.  We  produced  rLipL32  using  the  pRSET-C  vector  and  expressed  it  in  E.  coli  
BL21  (DE3)  competent  cells.  Under  native  conditions,  we  purified  2  mg  rLipL32  protein  from  500  ml  culture.  Analysis  using  
western  blotting  and  ELISA  showed  that  serum  from  the  bovine  positive  MAT  serovar  Hardjo  could  recognize  pure  rLipL32  
protein. This result confirms an earlier study that indicates that rLipL32 protein is a good antigen for Leptospirosis detection. The 
diagnostic assay using rLipL32 is safe because it does not use infectious bacteria as an antigen and because it is easy to perform 
in every diagnostic laboratory in Indonesia. However, further study is still required for field validation of the rLipL32 assay  aARTVET2098