02478     2200205   4500001002100000005001500021035002000036245022400056700006100280250003700341260000900378300002600387084001500413082001500428008003900443650003400482520169400516856004702210990001502257INLIS00000000001780820210804100912  a0010-0721002340  aTingkat Perlindungan Vaksin Komersial AI H5N1 Clade 2.1.3 terhadap Virus AI H5N1 clade 2.3.2 Asal Itik pada Ayam SPF dalam Kondisi Laboratorium /cIndriani, Risa; Dharmayanti, NLP Indi (Balai Besar Penelitian Veteriner)  aDharmayanti, NLP Indi (Balai Besar Penelitian Veteriner)  aJurnal Ilmu Ternak dan Veteriner  c2015  aVol. 20 (1), p.65-71.  aARTVET2081  aARTVET2081210804                |          | |   4aChicken, HPAI, Local, Vaccine  aHighly Pathogenic Avian Influenza (HPAI) subtype H5N1 clade 2.3.2 has infected chickens in farms, causing mortality and 
a decrease in egg production. Vaccination is one of the strategies to control disease of AI subtype H5N1. AI H5N1 clade 2.1.3 
vaccine is available commercially. The effectiveness of two vaccines of AI H5N1 clade 2.1.3 (product A and B), and AI H5N1 clade  2.3.2  (Sukoharjo)  against AI  H5N1  clade  2.3.2  (Sukoharjo)  virus  SPF  chickens  was  tested  in  laboratory.  Four  groups  of SPF chickens were used in this study, there were (1) vaccinated with H5N1 clade 2.1.3 (product A), (2) vaccinated with H5N1 clade  2.1.3  (product  B),  (3)  vaccinated  with  AI  H5N1  clade  2.3.2  and  (4)  unvaccinated  (as  a  control).  Each  vaccinated  group consisted of 10 chicken except 8 chicken for control group. SPF chicken were vaccinated with 1 dose of vaccine at 3 weeks olds, and then after 3 weeks post vaccination (at 6 weeks olds). All group of chicken were challenged with 106 EID50  per 0.1 ml via intranasal.  The  results  showed,  chicken  vaccinated  with  H5N1  clade  2.1.3  product  A  and  B  gave  100  and  80%  protection respectively, but showed challenged virus shedding, whereas vaccine of H5N1 clade 2.3.2 gave 100% protection from mortality and without virus shedding. Vaccines of AI H5N1 clade 2.1.3 product A was better than vaccine  product B, and when chicken vaccinated  against  H5N1  clade  2.3.2,  H5N1  clade  2.3.2  vaccine  was  the  best  to  be  used.  In  order  to  protect  chicken  from  AI subtype H5N1 clade 2.1.3 and 2.3.2 in the field, a bivalent vaccine of H5N1 clade 2.1.3 and 2.3.2 subtypes should be developed.  ahttp://dx.doi.org/10.14334/jitv.v20i1.1118  aARTVET2081