02000     2200205   4500001002100000005001500021035002000036245017100056700005700227250003700284260000900321300002600330084001500356082001500371008003900386650004000425520126700465856004701732990001501779INLIS00000000001780720210804091614  a0010-0721002339  aEfektivitas Metode PCR dan AGID dalam mendeteksi penyakit Enzootic Bovine Leucosis di Indonesia /cSaepulloh, M.; Sendow, Indrawati (Balai Besar Penelitian Veteriner)  aSendow, Indrawati (Balai Besar Penelitian Veteriner)  aJurnal Ilmu Ternak dan Veteriner  c2015  aVol. 20 (1), p.71-78.  aARTVET2080  aARTVET2080210804                |          | |   4aEnzootic Bovine Leucosis, Indonesia  aEnzootic  Bovine  Leucosis  (EBL)  is  one  of  viral  diseases  in  cattle  caused by  bovine  leukemia  virus  (BLV),  from Retroviridae. The virus can be detected using severals methods such as Polymerase Chain Reaction (PCR), while antibody can be detected using Agar Gel Immunodifussion (AGID). The aim of this experiment was to study the effectivity of PCR and AGID methods to detect enzootic bovine leukosis virus in Indonesia. Samples of peripheral blood leukocyte (PBL) were collected from cattles those with and without showing clinical signs. A total of 307 blood  and serum samples were  tested against BLV using PCR  and  AGID  tests,  while  21  semen  samples  which  were  from  similar  animals  for  blood  collection  were  collected  only  for PCR test. The results indicated that twelve cattles have positive  results with PCR test in PBL, but from those cattles  only seven were  positive  with  AGID.  On  the  other  hand,  the  PCR  did  not  detect  EBL  in  21  bovine  semen  samples  tested,  although  one sample gave positive result  with PCR in PBL. This results indicated that PCR method from blood samples  was  more sensitive than that AGID method. The PCR detection was also more sensitive for PBL than that for semen samples.  ahttp://dx.doi.org/10.14334/jitv.v20i1.1120  aARTVET2080