Indriani, Risa Dharmayanti, NLP Indi text 2013 Jurnal Biologi Indonesia Vol. 9 (2), p.159-166. An indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis (IBV). Antigen was prepared from whole virus of infectious bronchitis local isolate PTS-III serotype. Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA were determined 0.4pg/well, 1:2000 and 1:100, respectively. Correlation optical densities (OD) were compared with a standard commercial ELISA (R2=0.933). The developed ELISA has a better sensitivity to hemagglutination inhibition (HI) test. The developed local isolate ELISA can be used to detect antibody against infectious bronchitis virus and it is suitable for sample screening at the diagnostic laboratories. Indriani, Risa ; Dharmayanti, NLP Indi (Balai Besar Penelitian Veteriner) ELISA, antibody, chicken, IB PTS-III local isolate ARTVET2301 ARTVET2301 210806 20210806094518 INLIS000000000017794 Converted from MARCXML to MODS version 3.5 using MARC21slim2MODS3-5.xsl (Revision 1.106 2014/12/19)