01488     2200205   4500001002100000005001500021008003900036035002000075082001500095084001500110100001900125245020600144250002900350260000900379300002700388520077100415650005501186700002601241990001501267INLIS00000000001779420210806094518210806                |          | |    a0010-0721002326  aARTVET2301  aARTVET23011 aIndriani, Risa1 aEnzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Antibody in Chickens Using Local Isolate of PTS III /cIndriani, Risa ; Dharmayanti, NLP Indi (Balai Besar Penelitian Veteriner)  aJurnal Biologi Indonesia  c2013  aVol. 9 (2), p.159-166.  aAn indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis (IBV). Antigen was prepared from whole virus of infectious bronchitis local isolate PTS-III serotype. Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA were determined 0.4pg/well, 1:2000 and 1:100, respectively. Correlation optical densities (OD) were compared with a standard commercial ELISA (R2=0.933). The developed ELISA has a better sensitivity to hemagglutination inhibition (HI) test. The developed local isolate ELISA can be used to detect antibody against infectious bronchitis virus and it is suitable for sample screening at the diagnostic laboratories. 4aELISA, antibody, chicken, IB PTS-III local isolate1 aDharmayanti, NLP Indi  aARTVET2301